B6;129S2-Rbl2^tm1Tyj/J

Stock number: 008176
Research areas: Cancer Research, Developmental Biology Research

Description

Mice homozygous for this Rbl2 (retinoblastoma-like 2) targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities in this genetic background. No histologic abnormalities have been detected at birth or 2 months of age. No protein of normal size is detected by Western blot analysis of mouse embryonic fibroblasts which also show normal growth characteristics in culture. A very small amount of a truncated form of the protein has been detected by immunoprecipitation. This strain may be useful in studies of limb development or cancer.

Detailed description

Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities in this genetic background. No histologic abnormalities have been detected at birth or 2 months of age. The amount of mRNA in liver cells is reduced 5-fold. No protein of normal size is detected by Western blot analysis of mouse embryonic fibroblasts which also show normal growth characteristics in culture. A very small amount of a truncated form of the protein has been detected by immunoprecipitation and western blot analysis. This shorter form of p130 may come from alternative splicing. It is not known if this truncated form has any activity or not. This strain may be useful in studies of limb development or cancer.

Development

A targeting vector was designed to replace some sequences in exon 2 with a neomycin resistance cassette inserted in reverse orientation. The targeting vector was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Resultant chimeric male animals were crossed with C57BL/6 females. The strain was maintained on a mixed 129S2/SvPas and C57BL/6 background by the donating laboratory.

Allele

Symbol: Rbl2^tm1Tyj
Name: targeted mutation 1, Tyler Jacks
MGI accession ID: MGI:1857449
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Rbl2
Marker name: RB transcriptional corepressor like 2
Chromosome: 8
Strain of origin: 129S2/SvPas
Molecular note: A neomycin resistance cassette replaced sequences in a proximal exon of the Rbl2 gene. Rbl2 mRNA levels in liver cells of homozygous mutant animals were reduced more than five fold compared to wild-type. Immunoblot analysis did not detect RBL2 protein in embryonic fibroblasts or in adult liver of homozygous mutant mice.