Mice homozygous for this Rbl2 (retinoblastoma-like 2) targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities in this genetic background. No histologic abnormalities have been detected at birth or 2 months of age. No protein of normal size is detected by Western blot analysis of mouse embryonic fibroblasts which also show normal growth characteristics in culture. A very small amount of a truncated form of the protein has been detected by immunoprecipitation. This strain may be useful in studies of limb development or cancer.
Dr. Tyler Jacks, Massachusetts Institute of Technology
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Rbl2 | RB transcriptional corepressor like 2 |
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities in this genetic background. No histologic abnormalities have been detected at birth or 2 months of age. The amount of mRNA in liver cells is reduced 5-fold. No protein of normal size is detected by Western blot analysis of mouse embryonic fibroblasts which also show normal growth characteristics in culture. A very small amount of a truncated form of the protein has been detected by immunoprecipitation and western blot analysis. This shorter form of p130 may come from alternative splicing. It is not known if this truncated form has any activity or not. This strain may be useful in studies of limb development or cancer.
A targeting vector was designed to replace some sequences in exon 2 with a neomycin resistance cassette inserted in reverse orientation. The targeting vector was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Resultant chimeric male animals were crossed with C57BL/6 females. The strain was maintained on a mixed 129S2/SvPas and C57BL/6 background by the donating laboratory.
Allele Name | targeted mutation 1, Tyler Jacks |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | p130- |
Gene Symbol and Name | Rbl2, RB transcriptional corepressor like 2 |
Gene Synonym(s) | |
Strain of Origin | 129S2/SvPas |
Chromosome | 8 |
Molecular Note | A neomycin resistance cassette replaced sequences in a proximal exon of the Rbl2 gene. Rbl2 mRNA levels in liver cells of homozygous mutant animals were reduced more than five fold compared to wild-type. Immunoblot analysis did not detect RBL2 protein in embryonic fibroblasts or in adult liver of homozygous mutant mice. |
Mutations Made By | Dr. Tyler Jacks, Massachusetts Institute of Technology |
When maintained as a live colony, homozygotes may be bred.
When using the B6;129S2-Rbl2tm1Tyj/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008176 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Rbl2<tm1Tyj> |
Frozen Mouse Embryo | B6;129S2-Rbl2<tm1Tyj>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S2-Rbl2<tm1Tyj>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S2-Rbl2<tm1Tyj>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S2-Rbl2<tm1Tyj>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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