This strain may be useful in studies of regulatory T cell (Treg) development and function. Aged homozygous mice may show signs of obesity, supporting a proposed involvement of the gene in feeding and emotional behaviors. Naive CD4 T cells display a marginally reduced tendency to induce forkhead box P3 (Foxp3) in response to transforming growth factor, beta 1 (TGF-beta, Tgfb1) stimulation in vitro.
Alexander Rudensky, Memorial Sloan Kettering Cancer Center
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Gpr83 | G protein-coupled receptor 83 |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Gene product (mRNA) is not detected by quantitative RT-PCR analysis of T cells isolated from homozygous animals. Naive CD4 T cells display a marginally reduced tendency to induce forkhead box P3 (Foxp3) in response to transforming growth factor, beta 1 (TGF-beta, Tgfb1) stimulation in vitro. This strain may be useful in studies of regulatory T cell (Treg) development and function. Aged homozygous mice may show signs of obesity, supporting a proposed involvement of the gene in feeding and emotional behaviors.
A targeting vector was designed to place loxP sites on either side of the exon3/exon4 segment and introduce an FRT-flanked neomycin resistance cassette to intron 4. The construct was electroporated into 129X1/SvJ x 129S1/Sv-p+ Tyr+ KitlSl-J/J-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 background FLP and Cre deleter mice to remove the neomycin cassette and the exon 3/exon 4 segment, respectively.
Allele Name | targeted mutation 1.1, Alexander Y Rudensky |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | GPR83- |
Gene Symbol and Name | Gpr83, G protein-coupled receptor 83 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 9 |
Molecular Note | A loxP element was inserted 347 bp upstream of exon 3, and a cassette containing a loxP site and a FRT-flanked neomycin cassette was inserted 703 bp downstream of exon 4. Chimeric mice were first mated to FLP deleter mice to remove the neomycin cassette, and subsequently to Cre-transgenic mice to remove the floxed alleles. Gene deletion was confirmed by lack of expression in regulatory T cells as determined by quantitative RT-PCR. |
Mutations Made By | Jeffrey Rasmussen, University of Washington |
When maintained as a live colony, heterozygotes may be bred. Homozygous females do not breed well, but homozygous males may be used.
When using the B6;129-Gpr83tm1.1Ayr/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008156 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Gpr83<tm1.1Ayr> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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