B6;129-Gpr83^tm1.1Ayr/J

Stock number: 008156
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research, Neurobiology Research

Description

This strain may be useful in studies of regulatory T cell (Treg) development and function. Aged homozygous mice may show signs of obesity, supporting a proposed involvement of the gene in feeding and emotional behaviors. Naive CD4 T cells display a marginally reduced tendency to induce forkhead box P3 (Foxp3) in response to transforming growth factor, beta 1 (TGF-beta, Tgfb1) stimulation in vitro.

Detailed description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Gene product (mRNA) is not detected by quantitative RT-PCR analysis of T cells isolated from homozygous animals. Naive CD4 T cells display a marginally reduced tendency to induce forkhead box P3 (Foxp3) in response to transforming growth factor, beta 1 (TGF-beta, Tgfb1) stimulation in vitro. This strain may be useful in studies of regulatory T cell (Treg) development and function. Aged homozygous mice may show signs of obesity, supporting a proposed involvement of the gene in feeding and emotional behaviors.

Development

A targeting vector was designed to place loxP sites on either side of the exon3/exon4 segment and introduce an FRT-flanked neomycin resistance cassette to intron 4. The construct was electroporated into 129X1/SvJ x 129S1/Sv-p⁺ Tyr⁺ Kitl^Sl-J/J-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 background FLP and Cre deleter mice to remove the neomycin cassette and the exon 3/exon 4 segment, respectively.

Allele

Symbol: Gpr83^tm1.1Ayr
Name: targeted mutation 1.1, Alexander Y Rudensky
MGI accession ID: MGI:3768257
Generation method: Targeted
Attributes: Null/Knockout
Synonyms: GPR83^-
Marker symbol: Gpr83
Marker name: G protein-coupled receptor 83
Marker synonyms: Gir; glucocorticoid-induced receptor; GPR72; RP105; RP39; RP82
Chromosome: 9
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A loxP element was inserted 347 bp upstream of exon 3, and a cassette containing a loxP site and a FRT-flanked neomycin cassette was inserted 703 bp downstream of exon 4. Chimeric mice were first mated to FLP deleter mice to remove the neomycin cassette, and subsequently to Cre-transgenic mice to remove the floxed alleles. Gene deletion was confirmed by lack of expression in regulatory T cells as determined by quantitative RT-PCR.