B6;SJL-Tg(THY1-SNCA*A30P)TS2Sud/J

Stock number: 008134
Product name: TS2, UTSD#1910
Research areas: Neurobiology Research

Description

The A30P mutation in this transgenic strain is associated with the development of familial Parkinson's disease. The onset of hind limb mobility problems occurs around 12 months of age (sometimes earlier), induced by a loss of motor neurons and associated with the formation of insoluble alpha synuclein aggregates. This strain may be useful in studies of Parkinson's disease.

Detailed description

The A30P mutation in this transgenic strain is associated with the development of familial Parkinson's disease. The onset of hind limb mobility problems occurs around 12 months of age (sometimes earlier), induced by a loss of motor neurons and associated with the formation of insoluble alpha synuclein aggregates. This strain may be useful in studies of Parkinson's disease. Hemizygous mice are viable and fertile.

Development

This transgenic strain carries the human A30P mutant SNCA (synuclein, alpha) gene under the control of a human THY1 (thymus cell antigen 1, theta) promoter. The transgenic vector was injected into B6SJLF1/J embryos. mice from founder line TS2 were reportedly backcrossed to C57BL/6 for approximately ten generations prior to sending to The Jackson Laboratory Repository. However, a 27 SNP (single nucleotide polymorphism) panel analysis performed on mutant mice rederived by The Jackson Laboratory Repository revealed that 3/27 loci are still segregating for SJL background alleles. This suggests that line TS2 was only backcrossed a few generations before arriving, and the mice are still on a mixed B6;SJL mixed genetic background.

Allele

Symbol: Tg(THY1-SNCA*A30P)TS2Sud
Name: transgene insertion TS2, Thomas C Sudhof
MGI accession ID: MGI:3767582
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(THY1-SNCA*A30P)TS2Sud
Marker name: transgene insertion TS2, Thomas C Sudhof
Chromosome: UN
Strain of origin: (C57BL/6J x SJL/J)F1
Expressed genes: SNCA,synuclein alpha,human
Molecular note: The transgene construct contains the human A30P mutant SNCA (synuclein, alpha) gene under the control of a human THY1 (thymus cell antigen 1, theta) promoter.