B6.NOD-Tg(Cd4-EGFP)1Lt/J

Stock number: 008126
Strain synonyms: B6.CD4-GFP
Research areas: Research Tools

Description

These CD4-GFP transgenic mice may be useful for fluorescent monitoring of T cells both in vivo and in vitro.

Detailed description

The donating investigator reports that mice homozygous for this CD4-GFP transgene are viable, fertile, normal in size and do not exhibit any gross physical or behavioral abnormalities. FACS analysis of splenic lymphocytes shows transgenic expression uniformly in 81% of inactivated or resting CD8⁺, 81% on inactivated or resting CD4⁺, and minimal expression in B cells (2.3%) and macrophages (1.5%). Antigen activated CD4⁺ T cells continue to express GFP, while activated CD8⁺ T cells lost GFP expression. These CD4-GFP transgenic mice may be useful for fluorescent monitoring of T cells both in vivo and in vitro.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described on a NOD/ShiLt genetic background. We will modify the strain description if necessary as published results become available.

Development

A transgenic construct containing the coding region of the enhanced green fluorescent protein, EGFP, gene under the control of the murine Cd4 promoter and enhancer was injected into fertilized NOD/ShiLt mouse eggs. Founder line 1 was mated to NOD/ShiLt prior to sibling mating. These mice were sent to the Type 1 Diabetes Repository (T1DR) at The Jackson Laboratory in 2007 (as Stock No. 005334). Upon arrival, some mice were additionally sent to The Induced Mutant Resource at The Jackson Laboratory and then backcrossed to C57BL/6J to generate this congenic strain (Stock No. 008126).

Allele

Symbol: Tg(Cd4-EGFP)1Lt
Name: transgene insertion 1, Edward Leiter
MGI accession ID: MGI:3720219
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Cd4-EGFP)1Lt
Marker name: transgene insertion 1, Edward Leiter
Chromosome: UN
Strain of origin: NOD/ShiLt
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: FACs analysis has detected expression of the transgene in 81% of inactivated or resting CD8⁺ T cells and CD4⁺ T cells, with minimal expression in B cells (2.3%) and macrophages (1.5%).
Molecular note: A construct containing an enhanced green florescent protein cDNA fused to the murine Cd4 promoter and enhancer was injected into NOD/ShiLt fertilized embryos. T-cells glow green. 80% of T-cells show GFP expression by FACS while B-cells and macrophages do not express the transgene.