These CD4-GFP transgenic mice may be useful for fluorescent monitoring of T cells both in vivo and in vitro.
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
The donating investigator reports that mice homozygous for this CD4-GFP transgene are viable, fertile, normal in size and do not exhibit any gross physical or behavioral abnormalities. FACS analysis of splenic lymphocytes shows transgenic expression uniformly in 81% of inactivated or resting CD8+, 81% on inactivated or resting CD4+, and minimal expression in B cells (2.3%) and macrophages (1.5%). Antigen activated CD4+ T cells continue to express GFP, while activated CD8+ T cells lost GFP expression. These CD4-GFP transgenic mice may be useful for fluorescent monitoring of T cells both in vivo and in vitro.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described on a NOD/ShiLt genetic background. We will modify the strain description if necessary as published results become available.
A transgenic construct containing the coding region of the enhanced green fluorescent protein, EGFP, gene under the control of the murine Cd4 promoter and enhancer was injected into fertilized NOD/ShiLt mouse eggs. Founder line 1 was mated to NOD/ShiLt prior to sibling mating. These mice were sent to the Type 1 Diabetes Repository (T1DR) at The Jackson Laboratory in 2007 (as Stock No. 005334). Upon arrival, some mice were additionally sent to The Induced Mutant Resource at The Jackson Laboratory and then backcrossed to C57BL/6J to generate this congenic strain (Stock No. 008126).
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | FACs analysis has detected expression of the transgene in 81% of inactivated or resting CD8+ T cells and CD4+ T cells, with minimal expression in B cells (2.3%) and macrophages (1.5%). |
Allele Name | transgene insertion 1, Edward Leiter |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | Cd4-GFP line 1; Cd4-GFP line1 |
Gene Symbol and Name | Tg(Cd4-EGFP)1Lt, transgene insertion 1, Edward Leiter |
Gene Synonym(s) | |
Promoter | Cd4, Cd4 molecule, rat |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | FACs analysis has detected expression of the transgene in 81% of inactivated or resting CD8+ T cells and CD4+ T cells, with minimal expression in B cells (2.3%) and macrophages (1.5%). |
Strain of Origin | NOD/ShiLt |
Chromosome | UN |
Molecular Note | A construct containing an enhanced green florescent protein cDNA fused to the murine Cd4 promoter and enhancer was injected into NOD/ShiLt fertilized embryos. T-cells glow green. 80% of T-cells show GFP expression by FACS while B-cells and macrophages do not express the transgene. |
Mutations Made By | Dr. Edward Leiter, The Jackson Laboratory |
Mutant mice were bred to C57BL/6J mice to generate this congenic strain. When maintaining the live congenic colony, hemizygous mice are bred with wildtype siblings or to C57BL/6J inbred mice.
When using the B6.NOD-Tg(Cd4-EGFP)1Lt/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008126 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Cd4-EGFP)1Lt/ |
Frozen Mouse Embryo | B6.NOD-Tg(Cd4-EGFP)1Lt/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.NOD-Tg(Cd4-EGFP)1Lt/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.NOD-Tg(Cd4-EGFP)1Lt/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.NOD-Tg(Cd4-EGFP)1Lt/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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