B6N.129S4(129S6)-Sst^tm1Ute/J

Stock number: 008117
Research areas: Cancer Research, Endocrine Deficiency Research, Immunology, Inflammation and Autoimmunity Research, Internal/Organ Research, Neurobiology Research

Description

Somatostatin deficient mice exhibit increased baseline plasma growth hormone, corticosterone and total ghrelin levels and impaired motor performance. Hippocampal amyloid beta 42 peptides levels are elevated. This mutant mouse strain may be useful in studies of gastric homeostasis, learning and memory and endocrinology.

Detailed description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by Northern blot, in situ hybridization or radioimmunoassay analysis of brain tissue. Homozygotes exhibit increased baseline plasma growth hormone, corticosterone and total ghrelin levels compared to wildtype. Mutant mice have impaired motor performance ability. Somatostatin-deficient mice have enlarged stomachs with an increased number of parietal cells and hyperchlorhydria. Hippocampal neprilysin activity is diminished. Compared to wildtype controls, amyloid beta 42 peptides levels are elevated in the hippocampus. This mutant mouse strain may be useful in studies of gastric homeostasis, learning and memory and endocrinology.

Development

A targeting vector containing the neomycin resistance gene was used to disrupt the last 30 base pairs of exon 1 and the first 39 base pairs of intron 1. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells, and correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting male chimeric animals were crossed to 129S6/SvEvTac females, and then backcrossed to C57BL/6NHsd for 10 generations before arriving at The Jackson Laboratory. The mice were then backcrossed to C57BL/6NJ (Stock No. 005304) for one generation during importation.

Allele

Symbol: Sst^tm1Ute
Name: targeted mutation 1, Ute Hochgeschwender
MGI accession ID: MGI:1888381
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Sst
Marker name: somatostatin
Chromosome: 16
Strain of origin: 129S4/SvJae
Molecular note: An SV40-tk-neo cassette was inserted, thereby deleting the last 30 bp of exon 1 and the first 39 basepairs of inton 1. Successful recombination was shown by Southern blot. Northern blot indicated lack of transcript and radioimmunoassay demonstrated the lack of protein from homozygous mutants.