Somatostatin deficient mice exhibit increased baseline plasma growth hormone, corticosterone and total ghrelin levels and impaired motor performance. Hippocampal amyloid beta 42 peptides levels are elevated. This mutant mouse strain may be useful in studies of gastric homeostasis, learning and memory and endocrinology.
Ute Hochgeschwender, Central Michigan University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Sst | somatostatin |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by Northern blot, in situ hybridization or radioimmunoassay analysis of brain tissue. Homozygotes exhibit increased baseline plasma growth hormone, corticosterone and total ghrelin levels compared to wildtype. Mutant mice have impaired motor performance ability. Somatostatin-deficient mice have enlarged stomachs with an increased number of parietal cells and hyperchlorhydria. Hippocampal neprilysin activity is diminished. Compared to wildtype controls, amyloid beta 42 peptides levels are elevated in the hippocampus. This mutant mouse strain may be useful in studies of gastric homeostasis, learning and memory and endocrinology.
A targeting vector containing the neomycin resistance gene was used to disrupt the last 30 base pairs of exon 1 and the first 39 base pairs of intron 1. The construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells, and correctly targeted ES cells were injected into C57BL/6 blastocysts.
The resulting male chimeric animals were crossed to 129S6/SvEvTac females, and then backcrossed to C57BL/6NHsd for 10 generations before arriving at The Jackson Laboratory. The mice were then backcrossed to C57BL/6NJ (Stock No. 005304) for one generation during importation.
Allele Name | targeted mutation 1, Ute Hochgeschwender |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Srif-; Sst-; Sst KO |
Gene Symbol and Name | Sst, somatostatin |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 16 |
Molecular Note | An SV40-tk-neo cassette was inserted, thereby deleting the last 30 bp of exon 1 and the first 39 basepairs of inton 1. Successful recombination was shown by Southern blot. Northern blot indicated lack of transcript and radioimmunoassay demonstrated the lack of protein from homozygous mutants. |
Mutations Made By | Ute Hochgeschwender, Central Michigan University |
When maintaining a live colony, homozygous mice may be bred together.
When using the B6N.129S4(129S6)-Ssttm1Ute/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008117 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Sst<tm1Ute> |
Frozen Mouse Embryo | B6N.129S4(129S6)-Sst<tm1Ute>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.129S4(129S6)-Sst<tm1Ute>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.129S4(129S6)-Sst<tm1Ute>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6N.129S4(129S6)-Sst<tm1Ute>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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