These mice display reduced extracellular regulated kinase (ERK) signaling, and have smaller and fewer dendritic spines. Behaviorally, these mice exhibit impaired spatial learning and fear extinction, making them a potentially useful animal model of psychiatric diseases such as major depressive disorder and post-traumatic stress disorder (PTSD).
Morgan Sheng, Massachusetts Institute of Technology
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Hemizygous animals are fertile, viable, and grossly normal in physical appearance and behavior. The transgene product is expressed in excitatory neurons of the postnatal forebrain. There is an increase in active protein, detected biochemically. Immunohistochemistry and immunoblotting show expression in mouse forebrain (including the hippocampus, cortex, thalamus, striatum, and amygdala). These mice display reduced extracellular regulated kinase (ERK) signaling, and have smaller and fewer dendritic spines. Behaviorally, these mice exhibit impaired spatial learning and fear extinction, making them a potentially useful animal model of psychiatric diseases such as major depressive disorder and post-traumatic stress disorder (PTSD).
A vector carrying the mouse Camk2a promoter and human RAP2a (G12V (V12) mutant) was injected into C57BL/6 blastocysts. Resultant transgenic pups were bred to wildtype mice to establish a unique line.
Expressed Gene | RAP2A, RAP2A, member of RAS oncogene family, human |
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Site of Expression | Forebrain (including the hippocampus, cortex, thalamus, striatum, and amygdala). |
Allele Name | transgene insertion 1, Morgan Sheng |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | |
Gene Symbol and Name | Tg(Camk2a-RAP2A*G12V)1Shng, transgene insertion 1, Morgan Sheng |
Gene Synonym(s) | |
Promoter | Camk2a, calcium/calmodulin-dependent protein kinase II alpha, mouse, laboratory |
Expressed Gene | RAP2A, RAP2A, member of RAS oncogene family, human |
Site of Expression | Forebrain (including the hippocampus, cortex, thalamus, striatum, and amygdala). |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | The vector contains the mouse Camk2a promoter directing expression of the human RAP2A (G12V (V12) mutant). Immunohistochemistry and immunoblotting show expression in mouse forebrain (including the hippocampus, cortex, thalamus, striatum, and amygdala). |
When maintained as a live colony, hemizygotes may be bred. Transgenic females produce litters less frequently than wildtypes, and have smaller litters. For best productivity, a quiet environment and nesting materials are recommended.
When using the C57BL/6-Tg(Camk2a-RAP2A*G12V)1Shng/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008107 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrierfor Tg(Camk2a-RAP2V12)1Shng |
Frozen Mouse Embryo | C57BL/6-Tg(Camk2a-RAP2A*G12V)1Shng/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Camk2a-RAP2A*G12V)1Shng/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Tg(Camk2a-RAP2A*G12V)1Shng/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Tg(Camk2a-RAP2A*G12V)1Shng/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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