This transgenic strain provides a Tet-On tool that allows doxycycline (dox) inducible expression of genes in cells where the human KRT14 promoter is active and may be useful in applications for hair and skin research.
Elaine Fuchs, The Rockefeller University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Transactivator) |
Mice that are hemizygous or homozygous for this transgenic insert are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express the reverse tetracycline-controlled transactivator (rtTA) protein under the control of the human keratin 14 (KRT14) gene promoter (active in embryonic and postnatal basal cells of the skin). When mated to a second transgenic strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the target gene may be regulated by the tetracycline analog, doxycycline (dox); in the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed. This strain provides a Tet-On tool that allows the inducible expression of genes in cells expressing KRT14 for hair and skin research.
A transgenic vector incorporating the human keratin 14 promoter/enhancer, rabbit beta globin 5' untranslated region (UTR) for stabilization of transcript, the reverse tetracycline regulatable transactivator protein (rtTA2S-M2-VP16), and the keratin 14 3' UTR was injected into FVB embryos. This strain has been maintained on an FVB background by the donating laboratory.
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
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Site of Expression | embryonic and postnatal basal cells of the skin |
Allele Name | transgene insertion F42, Elaine Fuchs |
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Allele Type | Transgenic (Transactivator) |
Allele Synonym(s) | K14rtTA; K14-rtTA |
Gene Symbol and Name | Tg(KRT14-rtTA)F42Efu, transgene insertion F42, Elaine Fuchs |
Gene Synonym(s) | |
Promoter | KRT14, keratin 14, human |
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
Site of Expression | embryonic and postnatal basal cells of the skin |
Strain of Origin | FVB |
Chromosome | UN |
Molecular Note | A transgenic vector incorporating the human keratin 14 promoter/enhancer, rabbit beta globin 5' untranslated region (UTR) for stabilization of transcript, the reverse tetracycline regulatable transactivator protein (rtTA2S-M2-VP16), and the keratin 14 3' UTR was injected into FVB embryos. |
Mutations Made By | Elaine Fuchs, The Rockefeller University |
When maintained as a live colony, hemizygotes may be bred to wildtype siblings or to FVB/NJ inbred mice.
When using the K14rtTA mouse strain in a publication, please cite the originating article(s) and include JAX stock #008099 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(KRT14-rtTA)F42Efu |
Frozen Mouse Embryo | FVB-Tg(KRT14-rtTA)F42Efu/J | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(KRT14-rtTA)F42Efu/J | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(KRT14-rtTA)F42Efu/J | $3373.50 |
Frozen Mouse Embryo | FVB-Tg(KRT14-rtTA)F42Efu/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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