B6;129P2-Or8a1^{tm43(Or8a1)Mom}/MomJ

Stock number: 008092
Product name: M71-M71-GFP
Research areas: Developmental Biology Research, Neurobiology Research, Research Tools, Sensorineural Research

Description

This allele expresses two copies of Or8a1 (formerly Olfr151) and one Tau-GFP reporter from one tricistronic message thereby doubling the expression level of OR8A1 in the olfactory sensory neurons that express this allele.

Detailed description

This allele expresses two copies of Or8a1 (formerly Olfr151) and one Tau-GFP reporter from one tricistronic message thereby doubling the expression level of OR8A1 in the olfactory sensory neurons that express this allele. Histology reported by Movahedi et al. (2016) showed that GFP-expressing axons always form glomeruli. However, when crossed with the Tau-RFP tagged Or8a1^tm27Mom allele (see Stock No. 006679) the GFP-expressing axons in these compound heterozygotes converge in the correct location but instead of forming one glomerulus they segregate and form compartmentalized glomeruli.

Development

This allele was generated in 129P2-derived ES cells. The chimera was bred to C57BL/6, and the line was then maintained by sibling intercrossing. Sperm was cryopreserved from homozygous males.

Allele

Symbol: Or8a1^{tm43(Or8a1)Mom}
Name: targeted mutation 43, Peter Mombaerts
MGI accession ID: MGI:5788157
Generation method: Targeted
Attributes: Reporter; Inserted expressed sequence
Marker symbol: Or8a1
Marker name: olfactory receptor family 8 subfamily A member 1
Chromosome: 9
Strain of origin: 129P2/OlaHsd
Expressed genes: Or8a1,olfactory receptor family 8 subfamily A member 1,mouse, laboratory; GFP,Green Fluorescent Protein,
Site of expression: Olfactory glomeruli.
Molecular note: An internal ribosome entry sequence (IRES) followed by a second sequence encoding Olfr151 followed by a second IRES followed by GFP was inserted just downstream of the endogenous coding sequence of Olfr151, resulting in tricistronic expression that yields 2 copies of Olfr151 and one copy of GFP to double the expression level of Olfr151 from one allele.