NOD.L-(D11Mit314-D11Mit339)(D11Mit364-D11Mit168)/McdfJ

Stock number: 008058
Product name: NOD.Lc11g and DR3g
Strain synonyms: NOD.Lc11g, DR3g
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

Stock No.'s 008053, 008054, 008055, 008056, 008057, 008058, 008059 and 008060, are the result of crossing segments of C57L/J derived Chromosome 11 into the NOD/Bdc background. These strains experience significantly reduced diabetes and are useful for identifying candidate genes within the Idd4 subloci.

Detailed description

This NOD congenic strain, commonly referred to as NOD.Lc11g or DR3g carries Chromosome 11 alleles, D11Mit314 (51Mb) through D11Mit339 (63Mb) and D11Mit364 (72Mb) through D11Mit168 (113Mb) derived from C57L/J, which includes candidate gene Csf2 and Stat5a/Stat5b/Stat3 as well as Idd4.1, Idd4.2. Diabetes incidence in this strain is reported to be about 21% in females compared to 70% in female NOD control mice. (Davoodi-Semiromi et al, 2007).

This strain is useful to further study the role of Csf2 and Stat5a/Stat5b/Stat3 alleles in autoimmune disorders; to identify candidate diabetes resistant genes in the Idd4.1 and Idd4.2 regions and to positionally clone the Idd4 diabetes resistant loci.

Development

This NOD congenic stock (NOD.Lc11g, DR3g) contains a genomic segment from C57L/J introgressed into Chromosome 11. Seven additional stocks (Stock No's 008054, 008055, 008056, 008057, 008058, 008059, and 008060) were developed from Stock No. 008053 through backcrossing to NOD once, followed by selective breeding. This model, Stock No. 008058, is a double congenic containing C57L/J congenic intervals D11Mit314 (51Mb) through D11Mit339 (63Mb) and D11Mit364 (72Mb) through DllMit168 (113Mb) encoding C57L/J derived Csf2, Idd4.1, Idd4.2 and Stat5a/Stat5b/Stat3 alleles. In 2008, the Type 1 Diabetes Resource received this stock at N6F3.

Allele

MGI accession ID: MGI:102279
Marker symbol: D11Mit314
Marker name: DNA Segment, Chr 11, Massachusetts Institute of Technology 314
Chromosome: 11

Allele

MGI accession ID: MGI:102283
Marker symbol: D11Mit339
Marker name: DNA Segment, Chr 11, Massachusetts Institute of Technology 339
Chromosome: 11

Allele

MGI accession ID: MGI:106936
Marker symbol: D11Mit364
Marker name: DNA Segment, Chr 11, Massachusetts Institute of Technology 364
Chromosome: 11

Allele

MGI accession ID: MGI:89054
Marker symbol: D11Mit168
Marker name: DNA segment, Chr 11, Massachusetts Institute of Technology 168
Chromosome: 11