NOD.L-(D11Mit314-Csf2)/McdfJ

Stock number: 008055
Product name: NOD.Lc11b and DR3b
Strain synonyms: DR3b, NOD.Lc11b
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

Stock No.'s 008053, 008054, 008055, 008056, 008057, 008058, 008059 and 008060, are the result of crossing segments of C57L/J derived Chromosome 11 into the NOD/Bdc background. These strains experience significantly reduced diabetes and are useful for identifying candidate genes within the Idd4 subloci.

Detailed description

This NOD congenic strain, commonly referred to as NOD.Lc11b or DR3b, carries a small C57L/J derived congenic interval on Chromosome 11, D11Mit314 (51Mb) through Csf2 (54Mb) that encodes a cluster of cytokine genes including candidate gene Csf2 (Idd4.3) and excludes all of the telomeric regions downstream of Csf2. Stat5a, Stat5b, Stat3, are of NOD origin. Csf2 production by macrophages is significantly lower that of NOD macrophages. Stat5 phosphorylation levels of NOD.Lc11b macrophages is significantly lower than NOD macrophages(Litherland et al, 2005). Diabetes incidence in females is reported to be about 30% compared to 70% in NOD control mice (Davoodi-Semiromi et al, 2007).

This strain is useful to further study the role of Csf2 and to identify other Idd4 candidate genes and their role in autoimmune diabetes.

Development

This NOD congenic stock (NOD.Lc11b, DR3b) contains a genomic segment from C57L/J introgressed into Chromosome 11. Seven additional stocks (Stock No's 008054, 008055, 008056, 008057, 008058, 008059, and 008060) were developed from Stock No. 008053 through backcrossing to NOD once and selective breeding. This model, Stock No. 008055, contains the smallest C57L/J congenic interval D11Mit314 (51Mb) through Csf2 (54Mb) containing the C57L/J derived Csf2 allele (Idd4.3 region) while retaining the NOD derived Stat5a/Stat5b/Stat3 alleles. The Type 1 Diabetes Resource received this stock in 2009.

Allele

MGI accession ID: MGI:102279
Marker symbol: D11Mit314
Marker name: DNA Segment, Chr 11, Massachusetts Institute of Technology 314
Chromosome: 11

Allele

MGI accession ID: MGI:1339752
Marker symbol: Csf2
Marker name: colony stimulating factor 2 (granulocyte-macrophage)
Marker synonyms: CSF; Csfgm; GMCSF; Gm-csf; MGI-IGM
Chromosome: 11