NOD.L-(D11Mit314-D11Mit339)/McdfJ

Stock number: 008054
Product name: NOD.Lc11a and DR3a
Strain synonyms: DR3a, NOD.Lc11a
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

Stock No.'s 008053, 008054, 008055, 008056, 008057, 008058, 008059 and 008060 are the result of crossing segments of C57L/J derived Chromosome 11 into the NOD/Bdc background. These strains experience significantly reduced diabetes and are useful for identifying candidate genes within the Idd4 subloci.

Detailed description

This NOD congenic strain, commonly referred to as NOD.Lc11a or DR3a, carries a C57L/J derived congenic interval on Chromosome 11, D11Mit51 (51Mb) through D11Mit339 (63Mb) that includes the Idd4.3 region, containing candidate gene Csf2, and the Idd4.5 region. Stat5a, Stat5b, Stat3. Candidate genes in the Idd4.4 region along with Idd4.1, 4.2 regions are of NOD origin. Diabetes incidence in females is reported to be about 39% compared to 70% in NOD control mice. (Davoodi-Semiromi et al, 2007).

This strain is useful to further study the role of Csf2 and to identify other Idd4 candidate genes and their role in autoimmune diabetes.

Development

This NOD congenic stock contains a genomic segment from C57L/J introgressed into Chromosome 11. Seven additional stocks (Stock No's 008054, 008055, 008056, 008057, 008058, 008059, and 008060) were developed from Stock No. 008053 through backcrossing to NOD once and selective breeding. This model, Stock No. 008054, contains a small C57L/J congenic interval D11Mit314 (51Mb) through D11Mit339 (63Mb) containing the C57L/J derived Csf2 allele (Idd4.3 region) while retaining the NOD derived Stat5a/Stat5b/Stat3 alleles. In 2008, the Type 1 Diabetes Resource received this stock at N6F16.

Allele

MGI accession ID: MGI:96406
Marker symbol: Idd4
Marker name: insulin dependent diabetes susceptibility 4
Marker synonyms: Idd-4
Chromosome: 11

Allele

MGI accession ID: MGI:102283
Marker symbol: D11Mit339
Marker name: DNA Segment, Chr 11, Massachusetts Institute of Technology 339
Chromosome: 11

Allele

MGI accession ID: MGI:102279
Marker symbol: D11Mit314
Marker name: DNA Segment, Chr 11, Massachusetts Institute of Technology 314
Chromosome: 11