This transgene provides a control for that used in stocks #007980 and #007981 for investigating the promoter elements important in olfactory receptor expression. It encodes the same full coding sequence of Olfr16 with an IRES-tauGFP just after the stop codon resulting in bicistronic expression. This coding sequence is under the control of the 405 base pairs of regulatory sequence just upstream of the Olfr16 transcription start site and 9 equally spaced repeats of a mutated homeodomain binding sequence, upstream of this promoter. Each of the homeodomain sequences has two A to G mutations disrupting the binding site. These mutations in the homeobox domains reverse the massive increase in the number of cells expressing this olfactory receptor found in the transgenics with intact homeobox domains. The expression pattern for this transgenic instead resembles that of wild-type endogenous OLFR16 expression.

The inserted transgene in these mice contains a mutated homeodomain binding sequence upstream of the promoter for Olfr16 that results in an expression pattern resembling that of wild-type endogenous OLFR16 expression.

Typically mice are recovered in 12-16 weeks. Contact Customer Service to place an order or for more information.

Typically mice are recovered in 12-16 weeks. <a target="_blank" href="https://www.jax.org/jax-mice-and-services/customer-support" rel="noreferrer">Contact Customer Service</a> to place an order or for more information.