The inserted transgene in these mice contains a mutated homeodomain binding sequence upstream of the promoter for Olfr16 that results in an expression pattern resembling that of wild-type endogenous OLFR16 expression.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
This transgene provides a control for that used in stocks #007980 and #007981 for investigating the promoter elements important in olfactory receptor expression. It encodes the same full coding sequence of Olfr16 with an IRES-tauGFP just after the stop codon resulting in bicistronic expression. This coding sequence is under the control of the 405 base pairs of regulatory sequence just upstream of the Olfr16 transcription start site and 9 equally spaced repeats of a mutated homeodomain binding sequence, upstream of this promoter. Each of the homeodomain sequences has two A to G mutations disrupting the binding site. These mutations in the homeobox domains reverse the massive increase in the number of cells expressing this olfactory receptor found in the transgenics with intact homeobox domains. The expression pattern for this transgenic instead resembles that of wild-type endogenous OLFR16 expression.
This transgenic was generated by pronuclear microinjection into (C57BL/6J x CBA)F2 and was maintained on this segregating background until sperm was cryopreserved from hemizygous males.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | GFP is expressed in olfactory sensory neurons. |
Allele Name | transgene insertion 11, Peter Mombaerts |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | 9xmHD-TgdeltaMOR23-IRES-tauGFP |
Gene Symbol and Name | Tg(H*/Olfr16-GFP)11Mom, transgene insertion 11, Peter Mombaerts |
Gene Synonym(s) | |
Promoter | Olfr16, olfactory receptor 16, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP is expressed in olfactory sensory neurons. |
Strain of Origin | (C57BL/6J x CBA)F2 |
Chromosome | UN |
Molecular Note | As an experimental control a construct comprised of a stretch of 9 repeats of a mutated homeodomain binding sequence, having two A to G mutations disrupting each binding site, placed upstream of a minigene with 405 base pairs upstream of the transcription start site of Olfr16, an intact Olfr16 coding sequence, and an IRES-tauGFP cassette just after the stop codon was microinjected into (C57BL/6J x CBA)F2 pronuclei. |
When using the MOR23 Tg line 11 mouse strain in a publication, please cite the originating article(s) and include JAX stock #007986 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Carrier of Tg(H*/Olfr16-GFP)11Mom |
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