The inserted transgene in these mice contains the HD binding sequence upstream of the Olfr16 transcription start site that results in a massive increase of olfactory receptor cells with this olfactory receptor.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
This transgene encodes the full coding sequence of Olfr16 with an IRES-tauGFP just after the stop codon resulting in bicistronic expression. This coding sequence is under the control of 9 equally spaced repeats of the HD binding sequence and the 405 base pairs of regulatory sequence just upstream of the Olfr16 transcription start site. A massive increase is found in the number of cells that express this olfactory receptor, relative to the endogenous expression pattern, but the pattern of expression remains predominantly in the dorsal main olfactory epithelium, with some transgenic lines having additional narrow ventral domain expression.
This transgenic was generated by pronuclear microinjection into (C57BL/6J x CBA)F2 and was maintained on this segregating background until sperm was cryopreserved from hemizygous males.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | GFP is expressed in olfactory sensory neurons. |
Allele Name | transgene insertion 3, Peter Mombaerts |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | 9xHD-Tgdelta-MOR23-IRES-GFP |
Gene Symbol and Name | Tg(H/Olfr16-GFP)3Mom, transgene insertion 3, Peter Mombaerts |
Gene Synonym(s) | |
Promoter | Olfr16, olfactory receptor 16, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | GFP is expressed in olfactory sensory neurons. |
Strain of Origin | (C57BL/6J x CBA)F2 |
Chromosome | UN |
Molecular Note | A construct comprised of a stretch of 9 equally spaced repeats of the homeodomain binding sequence upstream of a minigene with 405 base pairs upstream of the transcription start site of Olfr16, an intact Olfr16 coding sequence, and and IRES-tauGFP cassette just after the stop codon was microinjected into (C57BL/6J x CBA)F2 pronuclei. |
When using the MOR23 Tg line III mouse strain in a publication, please cite the originating article(s) and include JAX stock #007979 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Carrier or Non Carrier of Tg(H/Olfr16-GFP)3Mom |
Frozen Mouse Embryo | B6;CBA-Tg(H/Olfr16-GFP)3Mom/MomJ | $2595.00 |
Frozen Mouse Embryo | B6;CBA-Tg(H/Olfr16-GFP)3Mom/MomJ | $2595.00 |
Frozen Mouse Embryo | B6;CBA-Tg(H/Olfr16-GFP)3Mom/MomJ | $3373.50 |
Frozen Mouse Embryo | B6;CBA-Tg(H/Olfr16-GFP)3Mom/MomJ | $3373.50 |
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