B6;CBA-Tg(Olfr713-taulacZ)4Mom/MomJ

Stock number: 007976
Product name: P3 Tg line IV
Research areas: Neurobiology Research, Research Tools

Description

These mice carry a transgene that encodes tau-linked-LacZ under the control of a minimal promoter upstream of the Olfr713 transcription start site. LacZ is expressed in place of any olfactory receptor sequence.

Detailed description

This transgene encodes tau-linked-LacZ under the control of a minimal promoter comprised of 143 base pairs upstream of the Olfr713 transcription start site and 163 base pairs of the 5' nontranslated exon 1. It does not drive expression of any olfactory receptor sequence. It is useful in mapping promoter elements important for olfactory receptor expression and the related olfactory sensory neuron development. Histology shows mosaic expression of LacZ in the ventral main olfactory epithelium.

Development

This transgenic was generated by pronuclear microinjection into (C57BL/6J x CBA)F2 and was maintained on this segregating background until sperm was cryopreserved from hemizygous males.

Allele

Symbol: Tg(Olfr713-taulacZ)4Mom
Name: transgene insertion 4, Peter Mombaerts
MGI accession ID: MGI:4850127
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Olfr713-taulacZ)4Mom
Marker name: transgene insertion 4, Peter Mombaerts
Chromosome: UN
Strain of origin: (C57BL/6J x CBA)F2
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ shows mosaic expression in the ventral main olfactory epithelium.
Molecular note: A construct comprised of 143 base pairs upstream of the Olfr713 transcription start site followed by 163 base pairs of the 5' nontranslated exon 1, followed by a LacZpA reporter was used for pronuclear microinjection to dissect promoter elements.