B6;CBA-Tg(Olfr151-taulacZ)4Mom/MomJ

Stock number: 007972
Product name: M71 Tg line IV
Research areas: Neurobiology Research, Research Tools

Description

These mice carry a transgene that encodes tau-linked-LacZ under the control of a minimal promoter upstream of the Olfr151 transcription start site. LacZ is expressed in place of any olfactory receptor sequence.

Detailed description

This transgene encodes tau-linked-LacZ under the control of a minimal promoter comprised of 161 base pairs upstream of the Olfr151 transcription start site and 176 base pairs of the 5' nontranslated exon 1. It does not drive expression of any olfactory receptor sequence. It is useful in mapping promoter elements important for olfactory receptor expression and the related olfactory sensory neuron development. Histology shows LacZ activity spread over a large domain of the dorsal aspect of the medial half-bulb, not coalescing into glomeruli.

Development

This transgenic was generated by pronuclear microinjection into (C57BL/6J x CBA)F2 and was maintained on this segregating background until sperm was cryopreserved from hemizygous males.

Allele

Symbol: Tg(Olfr151-taulacZ)4Mom
Name: transgene insertion 4, Peter Mombaerts
MGI accession ID: MGI:4850101
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Olfr151-taulacZ)4Mom
Marker name: transgene insertion 4, Peter Mombaerts
Chromosome: UN
Strain of origin: (C57BL/6J x CBA)F2
Expressed genes: lacZ,beta-galactosidase,E. coli
Site of expression: lacZ is expressed over a large domain of the dorsal aspect of the medial half-bulb.
Molecular note: A construct comprised of 161 base pairs upstream of the Olfr151 transcription start site, which includes one homeodomain and one OLF/EBP binding site, followed by the entire 5' nontranslated exon 1, which is 176 base pairs, followed by a LacZpA reporter was used for pronuclear microinjection to dissect promoter elements.