This strain functions as a reporter strain for Smn1 (survival motor neuron 1) in the heterozygous state. Exons 1 through 8 of the mouse Smn1 gene (12.8 kb) were replaced with lacZ and an FRT site remaining from the deletion of a selection marker. This allele is a functional null and homozygous animals are embryonic lethal.
Andrew Murphy, Regeneron Pharmaceuticals, Inc.
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Smn1 | survival motor neuron 1 |
This strain functions as a reporter strain for Smn1 (survival motor neuron 1) in the heterozygous state. Exons 1 through 8 of the mouse Smn1 gene (12.8 kb) were replaced with lacZ and an FRT site remaining from the deletion of a selection marker. This allele is a functional null and homozygous animals are embryonic lethal.
Importation of this model was supported by the Spinal Muscular Atrophy Foundation.
Exons 1 through 8 of the mouse Smn1 (survival motor neuron 1) gene (12.8 kb) were replaced with lacZ and an FRT site remaining from the deletion of a selection marker. 129S6/SvEvTac and C57BL/6Tac derived F1H4 ES cells were used. The mice were then backcrossed to FVB/NJ for at least 5 generations.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression |
Allele Name | targeted mutation 2, Andrew Murphy |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Smn allele A; Smn1KI; Smn1KO; Smn1lacZ |
Gene Symbol and Name | Smn1, survival motor neuron 1 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Strain of Origin | (129S6/SvEvTac x C57BL/6NTac)F1 |
Chromosome | 13 |
Molecular Note | Exons 1 through 8 of the mouse Smn1 (survival motor neuron 1) gene were replaced with lacZ and an FRT site remaining from the deletion of a selection marker. |
Mutations Made By | Nicole Graiff, Regeneron |
When maintaining a live colony, heterozygous mice may be bred to wildtype siblings, or to FVB/NJ inbred mice (Stock No. 001800). Homozygotes are embryonic lethal.
When using the FVB.Cg-Smn1tm2Mrph/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007955 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Smn1<tm2Mrph> |
Frozen Mouse Embryo | FVB.Cg-Smn1<tm2Mrph>/J | $2595.00 |
Frozen Mouse Embryo | FVB.Cg-Smn1<tm2Mrph>/J | $2595.00 |
Frozen Mouse Embryo | FVB.Cg-Smn1<tm2Mrph>/J | $3373.50 |
Frozen Mouse Embryo | FVB.Cg-Smn1<tm2Mrph>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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