FVB.Cg-Smn1^tm2Mrph/J

Stock number: 007955
Research areas: Developmental Biology Research, Neurobiology Research

Description

This strain functions as a reporter strain for Smn1 (survival motor neuron 1) in the heterozygous state. Exons 1 through 8 of the mouse Smn1 gene (12.8 kb) were replaced with lacZ and an FRT site remaining from the deletion of a selection marker. This allele is a functional null and homozygous animals are embryonic lethal.

Detailed description

This strain functions as a reporter strain for Smn1 (survival motor neuron 1) in the heterozygous state. Exons 1 through 8 of the mouse Smn1 gene (12.8 kb) were replaced with lacZ and an FRT site remaining from the deletion of a selection marker. This allele is a functional null and homozygous animals are embryonic lethal.

Importation of this model was supported by the Spinal Muscular Atrophy Foundation.

Development

Exons 1 through 8 of the mouse Smn1 (survival motor neuron 1) gene (12.8 kb) were replaced with lacZ and an FRT site remaining from the deletion of a selection marker. 129S6/SvEvTac and C57BL/6Tac derived F1H4 ES cells were used. The mice were then backcrossed to FVB/NJ for at least 5 generations.

Allele

Symbol: Smn1^tm2Mrph
Name: targeted mutation 2, Andrew Murphy
MGI accession ID: MGI:3794065
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Synonyms: Smn allele A; Smn1^KI; Smn1^KO; Smn1^lacZ
Marker symbol: Smn1
Marker name: survival motor neuron 1
Marker synonyms: BCD541; C-BCD541; GEMIN1; SMA; SMA@; SMA1; SMA2; SMA3; SMA4; Smn; SMNC; SMNT; T-BCD541; TDRD16A; TDRD16B
Chromosome: 13
Strain of origin: (129S6/SvEvTac x C57BL/6NTac)F1
Expressed genes: lacZ,beta-galactosidase,E. coli
Molecular note: Exons 1 through 8 of the mouse Smn1 (survival motor neuron 1) gene were replaced with lacZ and an FRT site remaining from the deletion of a selection marker.