Overview

Also Known As:NOD.A/J-Idd3

The 5 strains that comprise this NOD congenic set (Stock No.'s 007930, 007931, 007932, 007933, 007934) contain the Idd 3 region from non-NOD strains. Stocks containing the Idd 3 region from SWR/J, A/J, or CAST/EiJ are diabetes susceptible, while the congenic stocks containing the Idd 3 region derived from C57BL/6 or CZECHI/EiJ are diabetes resistant and produce more IL2 mRNA than the diabetes susceptible strains. These strains have been used to positionally clone Idd3 on Chr. 3 and continue to be valued to study the biological effects of variation of genes within the Idd3 region and for studying long-range chromosome remodeling.

Donating Investigator

Linda Wicker, University of Cambridge, UK

Genetic overview

Genetic Background	Generation

Idd3^A/J

Allele Type	Gene Symbol	Gene Name
QTL	Idd3	insulin dependent diabetes susceptibility 3

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Research Applications

Immunology, Inflammation and Autoimmunity Research
Diabetes and Obesity Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

This NOD congenic strain, commonly referred to as NOD.A/J-Idd3, is carrying Chr 3 alleles, SNPS rs168642 (18.178Mb) through rs13477164, (66.638Mb) derived from strain A/J including the insulin dependent diabetes susceptibility 3 loci (Idd3). Diabetes onset and incidence among mice of this strain is reported to be similar to NOD/MrkTac, with approximately 65% of the females becoming diabetic by 200 days of age. The congenic NOD.A/J-Idd3 females are predisposed to autoimmune ovarian dysgenesis. Thymocytes and splenocytes, either non-activated or activated of this T1D susceptible stock produce less Il2 mRNA than T1D protected strains (Stock No's. 007933 and 007934). Il2 molecules derived from the A/J strain are identical to NOD. This congenic strain, one of a set of 5 strains (007930, 007932, 007933 and 007934), has been useful to positionally clone Idd3 and to study the biological effects of variation of genes within the Idd3 region, specifically the Il2 and continue to be valued for studying long-range chromosome remodeling.

Development

An A/J (Stock No. 000646) congenic segment 47.69Mb in length is defined within SNPs rs168642 (18.178Mb) and rs13477164 (66.638Mb) that includes the Idd3 locus on Chr 3. Idd3 contains major candidate gene IL2 as well as IL21, Tenr, Cetn4 and Fgf2. The A/J congenic segment was introgressed onto the NOD/MrkTac background. This strain was produced by mating mice from NOD/MrkTac to A/J followed by 5 additional crosses to NOD/MrkTac with selection for Idd3 markers. Siblings carrying the A/J derived segment denoted by makers D3Mit329 through D3Mit274 were intercrossed to make homozygous. 5kSNP Chip Data, based on NCBI, build 36, identifies the congenic interval between SNPs rs6168642 (18.46Mb) and rs13477164 (66.15Mb) with no genetic contamination of other chromosomes. In 2007, the T1DR received this strain at generation N6F11.

Control Suggestions

Approximate Controls

001976 NOD/ShiLtJ

Additional Information

Considerations for Choosing Controls

Selected References

Yamanouchi J; Rainbow D; Serra P; Howlett S; Hunter K; Garner VE; Gonzalez-Munoz A; Clark J; Veijola R; Cubbon R; Chen SL; Rosa R; Cumiskey AM; Serreze DV; Gregory S; Rogers J; Lyons PA; Healy B; Smink LJ; Todd JA; Peterson LB; Wicker LS; Santamaria P. 2007. Interleukin-2 gene variation impairs regulatory T cell function and causes autoimmunity. Nat Genet 39(3):329-37PubMed: 17277778 MGI: J:120349

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Genetics

Idd3^A/J

Allele Symbol: Idd3^A/J

Allele Name	A/J
Allele Type	QTL
Allele Synonym(s)
Gene Symbol and Name	Idd3, insulin dependent diabetes susceptibility 3
Gene Synonym(s)
Strain of Origin	A/J
Chromosome	3
Molecular Note	This allele confers susceptibility to type 1 diabetes compared to C57BL/6J and CZECHI/EiJ. Diabetes onset and incidence among mice of this strain is reported to be similar to NOD/MrkTac mice.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
- Autoimmunity
  - Type 1 Diabetes
Diabetes and Obesity Research
- Type 1 Diabetes (IDDM) Analysis Strains
  - NOD/ShiLtJ Non-MHC Congenics

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Idd3^A/J/Idd3^A/J
NOD.A-Idd3

immune system phenotype

increased susceptibility to autoimmune diabetes

References

Yamanouchi J; Rainbow D; Serra P; Howlett S; Hunter K; Garner VE; Gonzalez-Munoz A; Clark J; Veijola R; Cubbon R; Chen SL; Rosa R; Cumiskey AM; Serreze DV; Gregory S; Rogers J; Lyons PA; Healy B; Smink LJ; Todd JA; Peterson LB; Wicker LS; Santamaria P. 2007. Interleukin-2 gene variation impairs regulatory T cell function and causes autoimmunity. Nat Genet 39(3):329-37PubMed: 17277778 MGI: J:120349

Additional - Idd3^A/J related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Genotyping resources and troubleshooting
Appearance
- albino
  Related Genotype: A/A Tyr^c/Tyr^c
Citation
When using the NOD.A/J-Idd3 mouse strain in a publication, please cite the originating article(s) and include JAX stock #007931 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Homozygous for Idd3<A/J>, 1 pair minimum

Related Products and Services

Frozen Mouse Embryo	NOD.A-Idd3<A/J>/MrkJ Frozen Embryos	$2595.00
Frozen Mouse Embryo	NOD.A-Idd3<A/J>/MrkJ Frozen Embryos	$2595.00
Frozen Mouse Embryo	NOD.A-Idd3<A/J>/MrkJ Frozen Embryos	$3373.50
Frozen Mouse Embryo	NOD.A-Idd3<A/J>/MrkJ Frozen Embryos	$3373.50

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Also Known As:NOD.A/J-Idd3

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Approximate Controls

Additional Information

Selected References

Idd3A/J

Allele Symbol: Idd3A/J

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Idd3A/J/Idd3A/JNOD.A-Idd3

References

Additional - Idd3A/J related

Genotyping Protocols

Appearance

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Idd3^A/J

Allele Symbol: Idd3^A/J

Genotype: Idd3^A/J/Idd3^A/J
NOD.A-Idd3

Additional - Idd3^A/J related