These mice harbor a targeted mutation of the H2-T23 gene (histocompatibility 2, T region locus 23 and may be useful in studying the role of this non-classical major histocompatibility complex (MHC) class Ib molecule in both CD8+ regulatory T cell activity and protection of activated T cells, as well as autoimmune diseases.
Harvey Cantor, Dana-Farber Cancer Institute (DFCI)
Mice homozygous for this Qa-1-deficient allele are viable and fertile. Targeted deletion of exons 1-3 of the H2-T23 gene is predicted to completely abrogate Qa-1 expression. Homozygous thymocytes and splenic T cells do not express Qa-1 following concanavalin A stimulation. Qa-1-deficient mice develop exaggerated secondary CD4+ T cell responses to foreign- and self-peptides; this phenotype is attributed to a lack of Qa-1-restricted CD8+ regulatory T cells normally associated with abrogating the secondary responses of CD4+ T cells. As such, homozygous mice are not protected from re-induction of experimental autoimmune encephalomyelitis (EAE) and are susceptible to autoimmune disease. These mutant mice may be useful in studying the role of H2-T23 (Qa-1), a non-classical major histocompatibility complex (MHC) class Ib molecule, in both CD8+ regulatory T cell activity and protection of activated T cells, as well as autoimmune diseases.
A targeting vector was designed to replace exons 1 through 3 (and also introduce a frameshift) of the targeted gene with a neomycin resistance cassette. Importantly, the donating investigator reports that the mouse DNA sequence used for gene targeting was derived from the genomic DNA of NM 010398, which is attributed to the H2-T23 gene (histocompatibility 2, T region locus 23). The targeting construct was electroporated into 129S6/SvEvTac-derived TC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6 females. Mutant mice were then backcrossed to C57BL/6 for 11 generations. Specifically, the donating investigator reports that all backcrossing in the two years prior to sending to The Jackson Laboratory were performed using C57BL/6 from Charles River (C57BL/6NCrl). Upon arrival, mice were bred with C57BL/6NJ inbred mice (Stock No. 005304) to establish the mutant colony.
|Allele Name||targeted mutation 1, Harvey Cantor|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Qa-1 KO; Qa-1-; Qa-1b-|
|Gene Symbol and Name||H2-T23, histocompatibility 2, T region locus 23|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||Exons 1 - 3 were replaced with a neo cassette via homologous recombination. This insertion introduced a frameshift. The absence of protein expression in activated thymocytes and splenic T cells was confirmed by flow cytometry.|
|Mutations Made By|| |
Harvey Cantor, Dana-Farber Cancer Institute (DFCI)
When maintaining a live colony, these mice may be bred as homozygotes.
When using the B6.129S6-H2-T23tm1Cant/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007907 in your Materials and Methods section.
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.
MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.