Overview

Also Known As: Ai9 or Ai9(RCL-tdT)

A C57BL/6J-congenic version of this strain is available as Stock No. 007909.

Ai9 is a Cre reporter allele that has a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato) - all inserted into the Gt(ROSA)26Sor locus. Ai9 mice express robust tdTomato fluorescence following Cre-mediated recombination.

Donating Investigator

Hongkui Zeng, Allen Institute for Brain Science

Genetic overview

Genetic Background	Generation

Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), Reporter)	Gt(ROSA)26Sor	gene trap ROSA 26, Philippe Soriano

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Research Applications

Research Tools
Neurobiology Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Ai9 mice homozygous for this Rosa-CAG-LSL-tdTomato-WPRE conditional allele are viable and fertile. A loxP-flanked STOP cassette is designed to prevent transcription of the red fluorescent protein variant tdTomato (see below). When bred to mice that express Cre recombinase, the resulting offspring will have the STOP cassette deleted in the cre-expressing tissue(s) - resulting in robust tdTomato fluorescence. Because this CAG promoter-driven reporter construct is inserted into the Gt(ROSA)26Sor locus, robust tdTomato expression is determined by which tissue(s) express Cre recombinase. These Ai9 mice are useful as a Cre reporter strain - expressing tdTomato fluorescence following Cre-mediated recombination.

Importantly, the donating investigator reports that very low levels of tdTomato expression may be present prior to introduction of Cre recombinase - but the tdTomato expression levels after Cre recombination are significantly greater than those baseline levels. As such, it is recommended that researchers include Cre-negative Ai9 controls to establish the baseline tdTomato levels in their experiments.

Of note, the FRT sites flanking the mutation allow for additional targeted replacement of the reporter sequences through Flp-mediated recombination if so desired. Similarly, the attB/attP-flanked selection cassette may be removed by introduction of the site-specific bacteriophage PhiC31 integrase if so desired.

For characterization information, see images at the Allen Institute for Brain Science website (Ai9 images).

The Allen Institute for Brain Science website has specific characterization information for several Cre Driver and Cre Reporter lines. Please see their website for images of Allen Institute for Brain Science experiments performed with all lines.

Of note, Ai9 mice are also available on a C57BL/6J-congenic background (see Stock No. 007909).

The Ai9 and Ai14 alleles are very similar in design - differing only in the presence or absence of an att site-flanked selection cassette at the 3' end of the targeted allele. Specifically, the Ai9 allele (Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}; Stock Nos. 007905/007909) is designed as Rosa26::CAG::frt::loxP-STOP-loxP::tdTomato::WPRE::polyA::attB-PGK-frt-neo-polyA-attP, whereas the Ai14 allele (Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}; Stock Nos. 007908/007914) is designed as Rosa26::CAG::frt::loxP-STOP-loxP::tdTomato::WPRE::polyA::attL.

Development

The Rosa-CAG-LSL-tdTomato-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), tdTomato sequence (a non-oligomerizing DsRed fluorescent protein variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus via electroporation of (129S6/SvEvTac x C57BL/6)F1-derived G4 embryonic stem (ES) cells. Correctly targeted ES cells (clone Ai9) were selected and chimeric males were bred to C57BL/6J females. The resulting Ai9 mice were then backcrossed to C57BL/6J for at least 1 generation prior to arrival at The Jackson Laboratory. These Ai9 mice on a B6;129S6 mixed genetic background are designated Stock No. 007905.

Expression Data

Expressed Gene	RFP, Red Fluorescent Protein, coral
Site of Expression	A floxed STOP prevents transcription of red fluorescent protein variant (tdTomato) until mice are bred to a Cre strain. Resulting offspring express tdTomato in a pattern dictated by the Cre promoter.

Control Suggestions

Approximate Controls

101045 B6129SF2/J

Additional Information

Considerations for Choosing Controls

Selected References

Madisen L; Zwingman TA; Sunkin SM; Oh SW; Zariwala HA; Gu H; Ng LL; Palmiter RD; Hawrylycz MJ; Jones AR; Lein ES; Zeng H. 2010. A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci 13(1):133-40PubMed: 20023653 MGI: J:155793

View All References

Genetics

Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}

Allele Symbol: Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}

Allele Name	targeted mutation 9, Hongkui Zeng
Allele Type	Targeted (Conditional ready (e.g. floxed), Reporter)
Allele Synonym(s)	targeted mutation 9, Hongkui Zeng; Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}
Gene Symbol and Name	Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano
Gene Synonym(s)	beta geo; ROSA26; R26; Gtrgeo26; Gtrosa26; Gtrgeo26; Gtrosa26; AV258896; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; SETD5-AS1; Thumpd3as1
Expressed Gene	RFP, Red Fluorescent Protein, coral
Site of Expression	A floxed STOP prevents transcription of red fluorescent protein variant (tdTomato) until mice are bred to a Cre strain. Resulting offspring express tdTomato in a pattern dictated by the Cre promoter.
Strain of Origin	(129S6/SvEvTac x C57BL/6NCrl)F1
Chromosome	6
Molecular Note	The Rosa-CAG-LSL-tdTomato-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), tdTomato sequence (a non-oligomerizing DsRed fluorescent protein variant with a 12 residue linker fusing two copies of the protein (tandem dimer)), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.
Mutations Made By	Hongkui Zeng, Allen Institute for Brain Science

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Genetics Research
  - Tissue/Cell Markers: transplantation marker for embryonic and adult tissue
  - Mutagenesis and Transgenesis
  - Tissue/Cell Markers: Cre-lox System
  - Tissue/Cell Markers: cell marker for bone marrow transplantation
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers
- Diabetes and Obesity Research
  - loxP
- FLP-FRT System
  - FRT-flanked Sequences
- Cre-lox System
  - loxP-flanked Sequences
  - loxP-flanked Sequences: Test/Reporter
- Developmental Biology Research
  - transplantation marker for embryonic and adult tissue
  - Cre-lox System
- Reproductive Biology Research
  - transplantation marker for embryonic and adult tissue
  - Cre-lox System
- Fluorescent Proteins
Neurobiology Research
- Cre-lox System
  - loxP-flanked Sequences
  - loxP-flanked Sequences: Test/Reporter
- Fluorescent protein expression in neural tissue

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Gt(ROSA)26Sor^{tm6(CAG-ZsGreen1)Hze}/Gt(ROSA)26Sor⁺
involves: 129S6/SvEvTac * C57BL/6NCr

normal phenotype

no abnormal phenotype detected
- Normal - mice are viable and fertile; used for cre reporter expression analysis

References

Madisen L; Zwingman TA; Sunkin SM; Oh SW; Zariwala HA; Gu H; Ng LL; Palmiter RD; Hawrylycz MJ; Jones AR; Lein ES; Zeng H. 2010. A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci 13(1):133-40PubMed: 20023653 MGI: J:155793

Additional References

Chakrabarti R; Celia-Terrassa T; Kumar S; Hang X; Wei Y; Choudhury A; Hwang J; Peng J; Nixon B; Grady JJ; DeCoste C; Gao J; van Es JH; Li MO; Aifantis I; Clevers H; Kang Y. 2018. Notch ligand Dll1 mediates cross-talk between mammary stem cells and the macrophageal niche. Science 360(6396)PubMed: 29773667 MGI: J:263857
Villahoz S; Yunes-Leites PS; Mendez-Barbero N; Urso K; Bonzon-Kulichenko E; Ortega S; Nistal JF; Vazquez J; Offermanns S; Redondo JM; Campanero MR. 2018. Conditional deletion of Rcan1 predisposes to hypertension-mediated intramural hematoma and subsequent aneurysm and aortic rupture. Nat Commun 9(1):4795PubMed: 30442942 MGI: J:268611
Chen Y; Ikeda K; Yoneshiro T; Scaramozza A; Tajima K; Wang Q; Kim K; Shinoda K; Sponton CH; Brown Z; Brack A; Kajimura S. 2019. Thermal stress induces glycolytic beige fat formation via a myogenic state. Nature 565(7738):180-185PubMed: 30568302 MGI: J:269371
Yaeger CE; Ringach DL; Trachtenberg JT. 2019. Neuromodulatory control of localized dendritic spiking in critical period cortex. Nature 567(7746):100-104PubMed: 30787434 MGI: J:274346
Sawen P; Eldeeb M; Erlandsson E; Kristiansen TA; Laterza C; Kokaia Z; Karlsson G; Yuan J; Soneji S; Mandal PK; Rossi DJ; Bryder D. 2018. Murine HSCs contribute actively to native hematopoiesis but with reduced differentiation capacity upon aging. Elife 7PubMed: 30561324 MGI: J:278611
Culemann S; Gruneboom A; Nicolas-Avila JA; Weidner D; Lammle KF; Rothe T; Quintana JA; Kirchner P; Krljanac B; Eberhardt M; Ferrazzi F; Kretzschmar E; Schicht M; Fischer K; Gelse K; Faas M; Pfeifle R; Ackermann JA; Pachowsky M; Renner N; Simon D; Haseloff RF; Ekici AB; Bauerle T; Blasig IE; Vera J; Voehringer D; Kleyer A; Paulsen F; Schett G; Hidalgo A; Kronke G. 2019. Locally renewing resident synovial macrophages provide a protective barrier for the joint. Nature 572(7771):670-675PubMed: 31391580 MGI: J:283709
Frandolig JE; Matney CJ; Lee K; Kim J; Chevee M; Kim SJ; Bickert AA; Brown SP. 2019. The Synaptic Organization of Layer 6 Circuits Reveals Inhibition as a Major Output of a Neocortical Sublamina. Cell Rep 28(12):3131-3143.e5PubMed: 31533036 MGI: J:284610

Additional - Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze} related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR:Gt(ROSA)26Sor(tdTomato-WPRE)
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, homozygous mice may be bred.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the Ai9 or Ai9(RCL-tdT) mouse strain in a publication, please cite the originating article(s) and include JAX stock #007905 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Gt(ROSA)26Sor<tm9(CAG-tdTomato)Hze>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Also Known As: Ai9 or Ai9(RCL-tdT)

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Gt(ROSA)26Sortm9(CAG-tdTomato)Hze

Allele Symbol: Gt(ROSA)26Sortm9(CAG-tdTomato)Hze

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/Gt(ROSA)26Sor+involves: 129S6/SvEvTac * C57BL/6NCr

normal phenotype

References

Additional References

Additional - Gt(ROSA)26Sortm9(CAG-tdTomato)Hze related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}

Allele Symbol: Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}

Genotype: Gt(ROSA)26Sor^{tm6(CAG-ZsGreen1)Hze}/Gt(ROSA)26Sor⁺
involves: 129S6/SvEvTac * C57BL/6NCr

Additional - Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze} related