These ROSA26-EGFP BAC transgenic mice may be useful for fluorescent labeling of cells and tissues both in vivo and in vitro, are suitable for use in histological preparations, and are appropriate as a source of fluorescently labeled donor cell populations (such as tracking hematopoietic cell fate in transplant recipients).
Andrew B. Leiter, University of Massachusetts Medical School
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Mice hemizygous for this ROSA26-EGFP BAC transgene are viable and fertile, with widespread EGFP expression in all organs and tissues analyzed. In addition, stable and uniform EGFP expression is observed in differentiated progeny of transplanted hematopoietic stem cells (HSC) isolated from ROSA26-EGFP mice. These ROSA26-EGFP BAC transgenic mice may be useful for fluorescent labeling of cells and tissues both in vivo and in vitro, are suitable for use in histological preparations, and are appropriate as a source of fluorescently labeled donor cell populations (such as tracking hematopoietic cell fate in transplant recipients).
The bacterial artificial chromosome (BAC) clone RP23-401D9 containing the mouse Gt(ROSA)26Sor gene (and 153 kb of upstream and 15 kb of downstream sequence) was modified by inserting a red-shifted variant Enhanced Green Fluorescent Protein (EGFP; Clontech No. 6029-1) cDNA and polyadenylation sequence at nucleotide 521 of the first exon of transcript 1. This ROSA26-EGFP BAC was microinjected into pronuclei of B6xB6D2F1 fertilized oocytes. Founders with a single copy of the transgene (founder line I1) were established and maintained by breeding with CD1 mice for at least 7 generations prior to arrival at The Jackson Laboratory.
Expressed Gene | GFP, Green Fluorescent Protein, |
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Site of Expression | EGFP is expressed in all organs and tissues analyzed. Stable and uniform EGFP expression is observed in differentiated progeny of transplanted hematopoietic stem cells (HSC) isolated from these mice. |
Allele Name | transgene insertion I1, Andrew B Leiter |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | ROSA26-EGFP; ROSA26-EGFP BAC |
Gene Symbol and Name | Tg(Gt(ROSA)26Sor-EGFP)I1Able, transgene insertion I1, Andrew B Leiter |
Gene Synonym(s) | |
Promoter | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano, mouse, laboratory |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP is expressed in all organs and tissues analyzed. Stable and uniform EGFP expression is observed in differentiated progeny of transplanted hematopoietic stem cells (HSC) isolated from these mice. |
Strain of Origin | C57BL/6 x (C57BL/6 x DBA/2)F1 |
Chromosome | UN |
Molecular Note | The bacterial artificial chromosome (BAC) clone RP23-401D9 containing the mouse Gt(ROSA)26Sor gene (and 153 kb of upstream and 15 kb of downstream sequence) was modified by inserting a red-shifted variant Enhanced Green Fluorescent Protein (EGFP; Clontech No. 6029-1) cDNA and polyadenylation sequence at nucleotide 521 of the first exon of transcript 1. Founders with a single copy of the transgene (founder line I1) were maintained on a CD-1 background. EGFP is ubiquitously and reproducibly expressed in all tissues and organs examined. Stable and uniform EGFP expression is observed in differentiated progeny of transplanted hematopoietic stem cells (HSC) isolated from ROSA26-EGFP mice. |
Mutations Made By | Andrew Leiter, University of Massachusetts Medical School |
When maintaining a live colony, hemizygous mice are bred to wildtype siblings.
When using the ROSA26-EGFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #007896 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non Carrierfor Tg(Gt(ROSA)26Sor-EGFP)I1Able |
Frozen Mouse Embryo | STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J | $3373.50 |
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