This allele of beta-2 microglobulin has the Venus yellow fluorescent reporter inserted in place of 64 base pairs of intron 1.
Peter Mombaerts, Max Planck Research Unit for Neurogenetics
This reporter allele of beta-2 microglobulin has the Venus yellow fluorescent reporter inserted in place of 64 base pairs of intron 1. This is likely a null allele, but protein or transcript analysis has not been done to validate this. This allele has been used in the heterozygous state as a reporter for beta-2 microglobulin expression (Bufe et al., 2019).
This allele was generated in 129P2/OlaHsd-derived E14 ES cells. 64 of the 67 nucleotides of exon 1 were replaced with the yellow fluorescent protein Venus, rabbit beta globin poly-A signal and the ACNf neomycin resistance cassette, which self-excises in the male germline. A male founder was bred to a C57BL/6J female and the line subsequently maintained by sibling breeding. Sperm was cryopreserved from heterozygous males and C57BL/6J oocytes are used for standard cryo-recovery.
|Allele Name||targeted mutation 1, Peter Mombaerts|
|Allele Type||Targeted (Reporter)|
|Gene Symbol and Name||B2m, beta-2 microglobulin|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||64 of the 67 nucleotides of exon 1 were replaced with the Venus yellow fluorescent protein and the ACNf neomycin selection cassette, which self excised from the male founder, resulting in a reporter allele and partial disruption of the coding sequence.|