B6;129P2-Vmn2r83^tm1Mom/MomJ

Stock number: 007886
Strain synonyms: V2rf4-Venus, V2rf4-IRES-tauVenus
Research areas: Developmental Biology Research, Research Tools, Sensorineural Research

Description

This reporter allele has a normal endogenous sequence but an IRES-tau-Venus inserted 3 nucleotides after the coding sequence, permitting bicistronic expression of the endogenous vomeronasal receptor and a tau-Venus reporter to mark the expressing neurons. This strain is useful in studies of vomeronasal receptor selection, neuronal connectivity and axonal development.

Detailed description

This reporter allele has a normal endogenous sequence but an IRES-tau-Venus inserted 3 nucleotides after the coding sequence, permitting bicistronic expression of the endogenous vomeronasal receptor and a tau-Venus reporter.

Development

This targeted insertion of a reporter was generated in 129P2/OlaHsd-derived E14 ES cells and the ACNF cassette, which had been co-inserted just downstream of the reporter, self-excised during germline transmission. The founder was bred to C57BL/6J. The resulting progeny were sibling intercrossed and sperm was cryopreserved from heterozygous males.

Allele

Symbol: Vmn2r83^tm1Mom
Name: targeted mutation 1, Peter Mombaerts
MGI accession ID: MGI:5907205
Generation method: Targeted
Attributes: Reporter
Marker symbol: Vmn2r83
Marker name: vomeronasal 2, receptor 83
Chromosome: 10
Strain of origin: 129P2/OlaHsd
Expressed genes: YFP,Yellow Fluorescent Protein,jellyfish; Vmn2r83,vomeronasal 2, receptor 83,mouse, laboratory
Site of expression: Vomeronasal sensory neurons
Molecular note: An IRES-tauVenus reporter cassette was inserted 3 nucelotides downstream of the coding sequence permitting bicistronic expression of the endogenous sequence and a tau-Venus reporter of expression.