B6;129-Rims2^tm1.2Schc/J

Stock number: 007855
Product name: RMF-Mouse Strain RIM2a conditional KO; UTSD#1902
Research areas: Neurobiology Research, Research Tools

Description

When bred to Cre transgenic strains, the loxP-flanked exon 5 is deleted by Cre expression to produce a "RIM2alpha" isoform null allele. This strain may be helpful in the analysis of the function of alpha-RIMs in specific brain regions.

Detailed description

Mice homozygous for this floxed targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. When bred to cre transgenic strains, the loxP-flanked exon 5 is deleted by cre expression to produce a "RIM2alpha" isoform null allele. This strain may be helpful in the analysis of the function of alpha-RIMs in specific brain regions.

Development

A targeting vector was designed to flank exon 5 of the gene with loxP sites and insert an FRT-flanked neomycin resistance cassette in reverse orientation in intron 4 (within the loxP sites). The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. Mice carrying the mutation were crossed with a FLP strain on a C57BL/6 and 129 mixed background driven by an actin promoter. The neomycin cassette was excised, leaving the floxed exon. This line was backcrossed two times to C57BL/6 by the donating laboratory.

Allele

Symbol: Rims2^tm1.2Schc
Name: targeted mutation 1.2, Susanne Schoch
MGI accession ID: MGI:3762950
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Rims2
Marker name: regulating synaptic membrane exocytosis 2
Chromosome: 15
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A targeting vector was designed to flank exon 5 of the gene with loxP sites and insert an FRT-flanked neomycin resistance cassette in reverse orientation in intron 4 (within the loxP sites). Mice carrying the mutation were crossed with a FLP strain on a C57BL/6 and 129 mixed background driven by an actin promoter. The neomycin cassette was excised, leaving the floxed exon.