B6;129-Erc1^tm1Sud/J

Stock number: 007854
Product name: E1X-Mouse Strain ELKS1 KO; UTSD#1898
Research areas: Developmental Biology Research

Description

Homozygous targeted mutation die early in embryonic development (~E9), but heterozygous animals appear normal. Detailed characterization of these mice has not been performed. These mice may be useful in studies of development.

Detailed description

Homozygous targeted mutation die early in embryonic development (~E9), but heterozygous animals appear normal. Detailed characterization of these mice has not been performed. These mice may be useful in studies of development.

Development

A targeting vector was designed to flank exon 1 of the gene with loxP sites and insert a FRT-flanked neomycin resistance cassette in intron 1 (within the loxP sites). The vector was introduced to (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells and injected into blastocysts. Resultant carrier mice were crossed with a germline Cre transgenic strain driven by a protamine 1 (Prm1) promoter on a mixed C57BL/6 and 129 background to remove both exon 1 and the neomycin cassette. This line was backcrossed two times to C57BL/6 by the donating laboratory.

Allele

Symbol: Erc1^tm1Sud
Name: targeted mutation 1, Thomas C Sudhof
MGI accession ID: MGI:3763189
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Erc1
Marker name: ELKS/RAB6-interacting/CAST family member 1
Chromosome: 6
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A targeting vector was designed to flank exon 3 of the gene with loxP sites and insert a FRT-flanked neomycin resistance cassette in intron 3 (within the loxP sites). Resultant carrier mice were crossed with a germline Cre transgenic strain driven by a protamine 1 (Prm1) promoter on a mixed C57BL/6 and 129 background to remove both exon 3 and the neomycin cassette.