This FLPo deleter strain may be useful for generating frt-FLP conditional mutations and serves as a source of FLPo-expressing primary cell lines.
Dr. Philippe Soriano, Mount Sinai School of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Recombinase-expressing) | Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano |
This FLPo deleter allele is a Gt(ROSA)26Sor knock-in containing a SA-Flpo-PGK-neo-polyA cassette. Homozygous ROSA26Flpo mice are viable and fertile, with widespread expression of the mouse codon-optimized FLP recombinase variant (FLPo) of the Saccharomyces cerevisiae FLP1 recombinase gene driven by the Gt(ROSA)26Sor promoter. The Gt(ROSA)26Sor promoter drives expression in a constitutive fashion from preimplantation onward. This is designed to achieve target gene recombination in most tissue types, including cells of the developing germ line. When bred with mice containing a FRT-flanked sequence of interest (with the FRT sites in the same orientation), FLP-mediated recombination will result in deletion of the FRT-flanked sequence(s) in the offspring. Flpo exhibits enhanced recombinase activity compared to Flpe in vivo. This FLPo deleter strain may be useful for generating frt-FLP conditional mutations and serves as a source of FLPo-expressing primary cell lines. The
A targeting vector containing a SA-Flpo-PGKneopA cassette was used to insert the mouse codon-optimized FLP recombinase (FLPo) coding sequence into a site located within intron 1 of the Gt(ROSA)26Sor locus. The construct was electroporated into 129S4/SvJaeSor derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129S4/SvJaeSor mice. Heterozygotes were intercrossed to generate homozygotes. Upon arrival at The Jackson Laboratory, the mice were crossed to 129S4/SvJaeJ (Stock No. 009104) at least once to establish the colony.
Expressed Gene | FLP, FLP recombinase, Saccharomyces cerevisiae |
---|---|
Site of Expression | Widespread. Target gene recombination can be achieved in most tissue types, including cells of the developing germ line, when bred with mice containing a FRT-flanked sequence of interest, |
Allele Name | targeted mutation 2, Philippe Soriano |
---|---|
Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | FLPo; Gt(ROSA)26Sortm2(FLPo)Sor; ROSA26FlpO |
Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano |
Gene Synonym(s) | |
Expressed Gene | FLP, FLP recombinase, Saccharomyces cerevisiae |
Site of Expression | Widespread. Target gene recombination can be achieved in most tissue types, including cells of the developing germ line, when bred with mice containing a FRT-flanked sequence of interest, |
Strain of Origin | 129S4/SvJaeSor |
Chromosome | 6 |
Molecular Note | A targeting vector containing a SA-Flpo-PGKneopA cassette was used to insert the mouse codon-optimized FLP recombinase (FLPo) coding sequence into a site located within intron 1 |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the FLPo (129 ROSA26Flpo) mouse strain in a publication, please cite the originating article(s) and include JAX stock #007844 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gt(ROSA)26Sor<tm2(FLP*)Sor> |
Frozen Mouse Embryo | 129S4/SvJae-Gt(ROSA)26Sor<tm2(FLP*)Sor>/J | $2595.00 |
Frozen Mouse Embryo | 129S4/SvJae-Gt(ROSA)26Sor<tm2(FLP*)Sor>/J | $2595.00 |
Frozen Mouse Embryo | 129S4/SvJae-Gt(ROSA)26Sor<tm2(FLP*)Sor>/J | $3373.50 |
Frozen Mouse Embryo | 129S4/SvJae-Gt(ROSA)26Sor<tm2(FLP*)Sor>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.