129S4/SvJae-Gt(ROSA)26Sor^tm2(FLP*)Sor/J

Stock number: 007844
Product name: FLPo (129 ROSA26Flpo)
Research areas: Research Tools

Description

This FLPo deleter strain may be useful for generating frt-FLP conditional mutations and serves as a source of FLPo-expressing primary cell lines.

Detailed description

This FLPo deleter allele is a Gt(ROSA)26Sor knock-in containing a SA-Flpo-PGK-neo-polyA cassette. Homozygous ROSA26Flpo mice are viable and fertile, with widespread expression of the mouse codon-optimized FLP recombinase variant (FLPo) of the Saccharomyces cerevisiae FLP1 recombinase gene driven by the Gt(ROSA)26Sor promoter. The Gt(ROSA)26Sor promoter drives expression in a constitutive fashion from preimplantation onward. This is designed to achieve target gene recombination in most tissue types, including cells of the developing germ line. When bred with mice containing a FRT-flanked sequence of interest (with the FRT sites in the same orientation), FLP-mediated recombination will result in deletion of the FRT-flanked sequence(s) in the offspring. Flpo exhibits enhanced recombinase activity compared to Flpe in vivo. This FLPo deleter strain may be useful for generating frt-FLP conditional mutations and serves as a source of FLPo-expressing primary cell lines. The

Development

A targeting vector containing a SA-Flpo-PGKneopA cassette was used to insert the mouse codon-optimized FLP recombinase (FLPo) coding sequence into a site located within intron 1 of the Gt(ROSA)26Sor locus. The construct was electroporated into 129S4/SvJaeSor derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129S4/SvJaeSor mice. Heterozygotes were intercrossed to generate homozygotes. Upon arrival at The Jackson Laboratory, the mice were crossed to 129S4/SvJaeJ (Stock No. 009104) at least once to establish the colony.

Allele

Symbol: Gt(ROSA)26Sor^tm2(FLP*)Sor
Name: targeted mutation 2, Philippe Soriano
MGI accession ID: MGI:3766925
Generation method: Targeted
Attributes: Recombinase-expressing
Synonyms: FLPo; Gt(ROSA)26Sor^tm2(FLPo)Sor; ROSA26FlpO
Marker symbol: Gt(ROSA)26Sor
Marker name: gene trap ROSA 26, Philippe Soriano
Marker synonyms: beta geo; Gtrgeo26; Gtrosa26; R26; ROSA26; SETD5-AS1; Thumpd3as1
Chromosome: 6
Strain of origin: 129S4/SvJaeSor
Expressed genes: FLP,FLP recombinase,Saccharomyces cerevisiae
Site of expression: Widespread. Target gene recombination can be achieved in most tissue types, including cells of the developing germ line, when bred with mice containing a FRT-flanked sequence of interest,
Molecular note: A targeting vector containing a SA-Flpo-PGKneopA cassette was used to insert the mouse codon-optimized FLP recombinase (FLPo) coding sequence into a site located within intron 1