B6;129P2-Aicda^tm172Mnz/J

Stock number: 007784
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

This targeted gene is expressed with a tandem FLAG-hemagglutinin (HA) tag on its amino terminus. Expression levels of the modified gene are equivalent to those found wild type B cells.

Detailed description

Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. The targeted gene is expressed with a tandem FLAG-hemagglutinin (HA) tag on its amino terminus. Expression levels of the modified gene are equivalent to those found wild type B cells.

Development

A targeting vector containing an FRT-flanked selection cassette (to be inserted between exons 1 and 2), and a tandem FLAG and hemagglutinin (HA) tag (to be inserted at the start site of exon 1) was introduced to E14 129P2/OlaHsd-derived embryonic stem (ES) cells. The mice carrying the mutations were bred to C57BL/6 background FLP mice to excise the selection cassette. This line was backcrossed to C57BL/6 two times by the donating laboratory.

Allele

Symbol: Aicda^tm172Mnz
Name: targeted mutation 172, Michel C Nussenzweig
MGI accession ID: MGI:3720569
Generation method: Targeted
Attributes: Reporter
Marker symbol: Aicda
Marker name: activation-induced cytidine deaminase
Chromosome: 6
Strain of origin: 129P2/OlaHsd
Expressed genes: HA tag,HA tag,; FLAG, FLAG octapeptide,
Site of expression: B lymphocytes
Molecular note: An FRT-flanked selection cassette was inserted between exons 1 and 2, and a tandem FLAG and hemagglutinin (HA) tag was inserted at the start site in exon 1. Flp-mediated recombination removed the neo cassette.