B6SJL-Tg(Dazl-hrGFP)4Gar/J

Stock number: 007732
Research areas: Reproductive Biology Research

Description

The mice express humanized Renilla Green Fluorescent Protein (hrGFP, Stratagene) specifically in germ cells of the testes under the control of the Dazl (deleted in azoospermia-like) promoter. Expression is highest in pachytene spermatocytes. No expression was found in the liver, heart, small intestine, large intestine, spleen, or kidney of these mice. This strain may be useful in studies of male fertility.

Detailed description

Mice homozygous for this transgene are viable and fertile and do not display any gross physical or behavioral abnormalities. The mice express humanized Renilla Green Fluorescent Protein (hrGFP, Stratagene) specifically in germ cells of the testes under the control of the Dazl (deleted in azoospermia-like) promoter. Expression is highest in pachytene spermatocytes. No expression was found in the liver, heart, small intestine, large intestine, spleen, or kidney of these mice. This strain may be useful in studies of male fertility.

Development

A 1.7 kb promoter fragment from just upstream of the translational start site of Dazl was amplified from B6SJLF1 genomic DNA by PCR. The PCR product was ligated into PCR Blunt (Invitrogen). The promoter fragment was cut out of PCR Blunt using NsiI and NotI, and then ligated into the NsiI and NotI sites of phrGFP (Stratagene) to generate pDazl-hrGFP. The Dazl-hrGFP construct was cut out of pDazl-hrGFP free of most other vector sequence using NsiI and MluI and used for pronuclear injection of B6SJLF1 oocytes to generate transgenic lines. This is founder line 4.

Allele

Symbol: Tg(Dazl-hrGFP)4Gar
Name: transgene insertion 4, David L Garbers
MGI accession ID: MGI:3722424
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Dazl-hrGFP)4Gar
Marker name: transgene insertion 4, David L Garbers
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F1
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: Humanized Renilla Green Fluorescent Protein (hrGFP) is expressed specifically in germ cells of the testes. Expression is highest in pachytene spermatocytes. No expression was found in the liver, heart, small intestine, large intestine, spleen, or kidney.
Molecular note: A 1.7 kb promoter fragment from just upstream of the translational start site of Dazl was used to drive expression of a humanized renilla GFP sequence (hrGFP). generate pDazl-hrGFP. The Dazl-hrGFP construct was used for pronuclear injection of (C57BL/6 x SJL)F1 oocytes to generate transgenic lines. Line 4 was established. GFP expression is highest in pachytene spermatocytes. No expression was found in the liver, heart, small intestine, large intestine, spleen, or kidney of these mice.