Mice that are homozygous for this targeted mutation have a perinatal lethal phenotype and die within hours of birth. At embryonic day 18.5 homozygous embryos exhibit reduced alveolar air space, thickened alveolar walls, small atelectactic areas and delayed lung development. This mutant mouse strain may be useful in studies of lung development and pulmonary physiology.
Annette Herscovics, McGill University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Man1a2 | mannosidase, alpha, class 1A, member 2 |
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygous mice have a perinatal lethal phenotype and die within hours of birth due to respiratory distress (cyanosis and alveolar hemorrhage). No gene product (mRNA) is detected by RT-PCR analysis of total RNA from homozygous embryonic tissues. At embryonic day 18.5 homozygous embryos exhibit reduced alveolar air space, thickened alveolar walls, small atelectactic areas and delayed lung development. This mutant mouse strain may be useful in studies of lung development and pulmonary physiology.
A targeting vector, pflox, containing sequence encoding exon 2 and loxP site flanked neomycin resistance and herpes simplex virus thymidine kinase genes was utilized in the construction of this mutant. This construct was electroporated into 129S4/SvJae derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre expression plasmid for the purpose of removing exon 2 and the selectable marker cassette. ES cells that had successfully undergone Cre recombination and no longer retained exon 2 or the selection cassette were were injected into C57BL/6 recipient blastocysts. Resulting chimeric animals were backcrossed to wildtype C57BL/6J mice.
Allele Name | targeted mutation 1.1, Annette Herscovics |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Man1B- |
Gene Symbol and Name | Man1a2, mannosidase, alpha, class 1A, member 2 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 3 |
Molecular Note | The floxed exon 2 in Man1a2tm1Ahe was removed via transient transfection with Cre recombinase. Excision of the floxed exon causes a frameshift and introduces a stop codon immediately following exon 1. RT-PCR confirmed the loss of gene expression in various tissues. |
Mutations Made By | Annette Herscovics, McGill University |
When maintaining a live colony, these mice are bred as heterozygotes. Homozygotes have a perinatal lethal phenotype and die within hours of birth.
When using the B6;129S4-Man1a2tm1.1Ahe/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007672 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-Type for Man1a2<tm1.1Ahe> |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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