B6.129S4(Cg)-Bmal1^tm1Weit/J

Stock number: 007668
Product name: Bmal1^lox
Research areas: Neurobiology Research, Research Tools

Description

These Bmal1 (basic helix-loop-helix ARNT like 1), formerly Arntl, floxed mutant mice may be useful in generating conditional mutations (whole-mouse or tissue-specific) to study the role of circadian clock/circadian rhythm in physiological and behavioral regulation.

Detailed description

Mice homozygous for this conditional (floxed) allele possess loxP sites flanking exon 8 of the targeted gene and are viable and fertile, with circadian behavioral rhythms indistinguishable from wildtype littermates. When bred to mice that express Cre recombinase, the resulting offspring will have the exon encoding the BMAL1 (formerly ARNTL) basic helix-loop-helix (bHLH) domain deleted in the cre-expressing tissue(s). These Bmal1-floxed mutant mice may be useful in generating conditional mutations (whole-mouse or tissue-specific) to study the role of circadian clock/circadian rhythm in physiological and behavioral regulation.

For example, when crossed to a strain expressing Cre recombinase in the retina (see Stock No. 005105), this mutant mouse strain may be useful in studies of the circadian clock of the retina.

Development

Using homologous recombination in a bacterial artificial chromosome (BAC, clone RPCI23-331K23), an FRT-flanked neo cassette (followed by a loxP site) was inserted immediately downstream of exon 8 of the targeted gene. An additional loxP site was added upstream of exon 8 when the targeting vector was excised from the BAC. This construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric mice were bred to Flpe-expressing mice (see Stock No. 003946) to excise the neo cassette. The resulting offspring were next bred to C57BL/6 congenic mutant mice (non-germline cre mice). After this, the donating laboratory reported the mice were selectively backcrossed to C57BL/6J inbred mice for at least 6 generations to contain only the conditional mutation (loxP-flanked exon 8 with a single remaining FRT site) prior to sending to The Jackson Laboratory in 2007. Upon arrival, the strain was bred to C57BL/6J at least one generation to establish our live colony. Subsequently, homozygous sperm (generation N7+N1F1) was cryoprererved in 2008.

Allele

Symbol: Bmal1^tm1Weit
Name: targeted mutation 1, Charles Weitz
MGI accession ID: MGI:3720808
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Synonyms: Bmal^f; Bmal1^lox; conditional Bmal
Marker symbol: Bmal1
Marker name: basic helix-loop-helix ARNT like 1
Marker synonyms: Arnt3; ARNTL; ARNTL1; bHLHe5; BMAL1c; JAP3; MOP3; PASD3; TIC
Chromosome: 7
Strain of origin: 129S4/SvJae
Molecular note: Using homologous recombination in a bacterial artificial chromosome (BAC, clone RPCI23-331K23), an FRT-flanked neo cassette (followed by a loxP site) was inserted immediately downstream of exon 8 of the targeted gene. An additional loxP site was added upstream of exon 8 when the targeting vector was excised from the BAC. Correctly targeted ES cells were injected into blastocysts and chimeric mice were bred to Flpe-expressing mice to excise the neo cassette.