When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene (widespread deletion of expression is lethal).
Dr. Richard A. Flavell, Yale University School of Medicine
These mice possess loxP sites on either side of the cytokine-responsive promoter region and exon 1 of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene (widespread deletion of expression is lethal).
For example, when crossed to a strain expressing interferon inducible Cre recombinase (see Stock No. 003556), this mutant mouse strain may be useful in studies of bone marrow nonhematopoietic cells.
When crossed to a strain expressing Cre recombinase in endothelial cells (see Stock No. 004128), this mutant mouse strain may be useful in studies of lymphocyte migration.
A targeting vector was designed to place loxP sites on either side of the promoter and exon 1 of the gene, and insert a lox-P-flanked neomycin resistance cassette in intron 1. The targeting vector was introduced to 129-derived embryonic stem (ES) cells. Homologous recombinant ES cells were injected into C57BL/6 blastocysts and chimeric males were bred to C57BL/6 females. Deletion of the floxed neomycin cassette was achieved by crossing the animals to a "splicer" Tet-Cre mouse maintained on a mixed C3H and C57BL/6 background. This strain has been backcrossed to C57BL/6 for 10 generations by the donating laboratory.
|Allele Name||targeted mutation 2, Richard A Flavell|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Vcam1, vascular cell adhesion molecule 1|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||The cytokine responsive promoter region and exon 1 were left flanked by single loxP site after a floxed neo cassette was excised by in vivo expression of cre recombinase. Normal protein levels were detected in homozygous mutant mice by analysis of dendritic cells obtained from the spleen.|
|Mutations Made By|| |
Dr. Richard Flavell, Yale University School of Medicine
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6.129(C3)-Vcam1tm2Flv/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007665 in your Materials and Methods section.