When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene (widespread deletion of expression is lethal).
Dr. Richard A. Flavell, Yale University School of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Vcam1 | vascular cell adhesion molecule 1 |
These mice possess loxP sites on either side of the cytokine-responsive promoter region and exon 1 of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene (widespread deletion of expression is lethal).
For example, when crossed to a strain expressing interferon inducible Cre recombinase (see Stock No. 003556), this mutant mouse strain may be useful in studies of bone marrow nonhematopoietic cells.
When crossed to a strain expressing Cre recombinase in endothelial cells (see Stock No. 004128), this mutant mouse strain may be useful in studies of lymphocyte migration.
A targeting vector was designed to place loxP sites on either side of the promoter and exon 1 of the gene, and insert a lox-P-flanked neomycin resistance cassette in intron 1. The targeting vector was introduced to 129-derived embryonic stem (ES) cells. Homologous recombinant ES cells were injected into C57BL/6 blastocysts and chimeric males were bred to C57BL/6 females. Deletion of the floxed neomycin cassette was achieved by crossing the animals to a "splicer" Tet-Cre mouse maintained on a mixed C3H and C57BL/6 background. This strain has been backcrossed to C57BL/6 for 10 generations by the donating laboratory.
Allele Name | targeted mutation 2, Richard A Flavell |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | vcam-1flox |
Gene Symbol and Name | Vcam1, vascular cell adhesion molecule 1 |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 3 |
Molecular Note | The cytokine responsive promoter region and exon 1 were left flanked by single loxP site after a floxed neo cassette was excised by in vivo expression of cre recombinase. Normal protein levels were detected in homozygous mutant mice by analysis of dendritic cells obtained from the spleen. |
Mutations Made By | Dr. Richard Flavell, Yale University School of Medicine |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the B6.129(C3)-Vcam1tm2Flv/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007665 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Vcam1<tm2Flv> |
Frozen Mouse Embryo | B6.129(C3)-Vcam1<tm2Flv>/J | $2595.00 |
Frozen Mouse Embryo | B6.129(C3)-Vcam1<tm2Flv>/J | $2595.00 |
Frozen Mouse Embryo | B6.129(C3)-Vcam1<tm2Flv>/J | $3373.50 |
Frozen Mouse Embryo | B6.129(C3)-Vcam1<tm2Flv>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.