B6.129(C3)-Vcam1^tm2Flv/J

Stock number: 007665
Research areas: Research Tools

Description

When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene (widespread deletion of expression is lethal).

Detailed description

These mice possess loxP sites on either side of the cytokine-responsive promoter region and exon 1 of the targeted gene. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific expression of the gene (widespread deletion of expression is lethal).

For example, when crossed to a strain expressing interferon inducible Cre recombinase (see Stock No. 003556), this mutant mouse strain may be useful in studies of bone marrow nonhematopoietic cells.

When crossed to a strain expressing Cre recombinase in endothelial cells (see Stock No. 004128), this mutant mouse strain may be useful in studies of lymphocyte migration.

Development

A targeting vector was designed to place loxP sites on either side of the promoter and exon 1 of the gene, and insert a lox-P-flanked neomycin resistance cassette in intron 1. The targeting vector was introduced to 129-derived embryonic stem (ES) cells. Homologous recombinant ES cells were injected into C57BL/6 blastocysts and chimeric males were bred to C57BL/6 females. Deletion of the floxed neomycin cassette was achieved by crossing the animals to a "splicer" Tet-Cre mouse maintained on a mixed C3H and C57BL/6 background. This strain has been backcrossed to C57BL/6 for 10 generations by the donating laboratory.

Allele

Symbol: Vcam1^tm2Flv
Name: targeted mutation 2, Richard A Flavell
MGI accession ID: MGI:2386556
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Vcam1
Marker name: vascular cell adhesion molecule 1
Chromosome: 3
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Molecular note: The cytokine responsive promoter region and exon 1 were left flanked by single loxP site after a floxed neo cassette was excised by in vivo expression of cre recombinase. Normal protein levels were detected in homozygous mutant mice by analysis of dendritic cells obtained from the spleen.