STOCK Gucy2c^tm1Gar/J

Stock number: 007660
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

Homozygotes are resistant to heat-stable enterotoxin (STa)-induced diarrhea and to enterotoxigenic bacteria that produce STa. Cholera toxin, however, causes diarrhea. These mice may be useful in studies of enteric infection.

Detailed description

Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. They are resistant to heat-stable enterotoxin (STa)-induced diarrhea and to enterotoxigenic bacteria that produce STa. Cholera toxin, however, causes diarrhea. Expression is not detected by Western blot analysis of intestinal mucosal preparations. These mice may be useful in studies of enteric infection.

Development

A targeting vector was designed to insert a neomycin resistance cassette under the control of a Pol II promoter (in the opposite transcriptional orientation) in exon 1 of the gene. The targeting vector was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Targeted ES cells were injected into C57BL/6 blastocysts. Male chimeras were crossed to C57BL/6J or Black Swiss females.

Allele

Symbol: Gucy2c^tm1Gar
Name: targeted mutation 1, David L Garbers
MGI accession ID: MGI:2665316
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Gucy2c
Marker name: guanylate cyclase 2c
Chromosome: 6
Strain of origin: 129S6/SvEvTac
Molecular note: Exon 1 was disrupted by a neomycin selection cassette inserted by homologous recombination. Protein was undetected by Western blot analysis of intestinal mucosal scrapings obtained from homozygous mutant mice. Total intestine mucosal guanylyl cyclase activity was reduced 16-fold relative to wild-type controls, whereas heat-stable enterotoxin (STa)-stimulable guanylyl cyclase activity was absent in mutant mice.