Homozygotes are resistant to heat-stable enterotoxin (STa)-induced diarrhea and to enterotoxigenic bacteria that produce STa. Cholera toxin, however, causes diarrhea. These mice may be useful in studies of enteric infection.
David L. Garbers, U.T. Southwestern Medical Center
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. They are resistant to heat-stable enterotoxin (STa)-induced diarrhea and to enterotoxigenic bacteria that produce STa. Cholera toxin, however, causes diarrhea. Expression is not detected by Western blot analysis of intestinal mucosal preparations. These mice may be useful in studies of enteric infection.
A targeting vector was designed to insert a neomycin resistance cassette under the control of a Pol II promoter (in the opposite transcriptional orientation) in exon 1 of the gene. The targeting vector was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Targeted ES cells were injected into C57BL/6 blastocysts. Male chimeras were crossed to C57BL/6J or Black Swiss females.
|Allele Name||targeted mutation 1, David L Garbers|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||GC-C -; Gcc-|
|Gene Symbol and Name||Gucy2c, guanylate cyclase 2c|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||Exon 1 was disrupted by a neomycin selection cassette inserted by homologous recombination. Protein was undetected by Western blot analysis of intestinal mucosal scrapings obtained from homozygous mutant mice. Total intestine mucosal guanylyl cyclase activity was reduced 16-fold relative to wild-type controls, whereas heat-stable enterotoxin (STa)-stimulable guanylyl cyclase activity was absent in mutant mice.|
|Mutations Made By|| |
David Garbers, U.T. Southwestern Medical Center
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the STOCK Gucy2ctm1Gar/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007660 in your Materials and Methods section.