Homozygotes are resistant to heat-stable enterotoxin (STa)-induced diarrhea and to enterotoxigenic bacteria that produce STa. Cholera toxin, however, causes diarrhea. These mice may be useful in studies of enteric infection.
David L. Garbers, U.T. Southwestern Medical Center
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Gucy2c | guanylate cyclase 2c |
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. They are resistant to heat-stable enterotoxin (STa)-induced diarrhea and to enterotoxigenic bacteria that produce STa. Cholera toxin, however, causes diarrhea. Expression is not detected by Western blot analysis of intestinal mucosal preparations. These mice may be useful in studies of enteric infection.
A targeting vector was designed to insert a neomycin resistance cassette under the control of a Pol II promoter (in the opposite transcriptional orientation) in exon 1 of the gene. The targeting vector was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Targeted ES cells were injected into C57BL/6 blastocysts. Male chimeras were crossed to C57BL/6J or Black Swiss females.
Allele Name | targeted mutation 1, David L Garbers |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | GC-C -; Gcc- |
Gene Symbol and Name | Gucy2c, guanylate cyclase 2c |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 6 |
Molecular Note | Exon 1 was disrupted by a neomycin selection cassette inserted by homologous recombination. Protein was undetected by Western blot analysis of intestinal mucosal scrapings obtained from homozygous mutant mice. Total intestine mucosal guanylyl cyclase activity was reduced 16-fold relative to wild-type controls, whereas heat-stable enterotoxin (STa)-stimulable guanylyl cyclase activity was absent in mutant mice. |
Mutations Made By | David Garbers, U.T. Southwestern Medical Center |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the STOCK Gucy2ctm1Gar/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007660 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Gucy2c<tm1Gar> |
Frozen Mouse Embryo | STOCK Gucy2c<tm1Gar>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Gucy2c<tm1Gar>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Gucy2c<tm1Gar>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Gucy2c<tm1Gar>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.