-Sperm morphology and motility are unaffected, but tests show that phosphatidylserine (PS) already exists in the outer membrane leaflet before sperm capacitation. When the zona pellucida (ZP) is removed from eggs, fertilization rates are normal; although when the extracellular matrix is intact, fewer spermatozoa bind tightly or penetrate the ZP, and fewer undergo acrosome reactions. This strain may be useful in studies of male fertility.
David L. Garbers, U.T. Southwestern Medical Center
Both male and female homozygotes are fertile, but the average litter sizes from homozygous males are slightly smaller than observed with wildtype males. Sperm morphology and motility are unaffected, but tests show that phosphatidylserine (PS) already exists in the outer membrane leaflet before sperm capacitation. When the zona pellucida (ZP) is removed from eggs, fertilization rates are normal; although when the extracellular matrix is intact, fewer spermatozoa bind tightly or penetrate the ZP, and fewer undergo acrosome reactions. Homozygous mice do not express protein as determined by Western blot analysis of testis tissues and immunostaining of mature spermatozoa. This published information is based on studies of mixed background C57BL/6 and 129S6 background animals; the line offered here is on a pure 129S6 background. This strain may be useful in studies of male fertility.
A targeting vector was designed to replace exons 4-14, encoding the first four transmembrane segments, with a neomycin resistance cassette. The vector was electroporated into 129S6/SvEvTac-derived SM1 embryonic stem (ES) cells. Chimeric mice were crossed with 129S6/SvEvTac animals.
|Allele Name||targeted mutation 1, David L Garbers|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Atp8b3, ATPase, class I, type 8B, member 3|
|Strain of Origin||129S/SvEv|
|Molecular Note||A genomic fragment encompassing exons 4 through 14 was replaced with a neomycin selection cassette. The deleted region encoded the first four transmembrane domains of the protein. No protein was detected in homozygous mutant mice by Western blot analysis of testes membrane extracts or by immunostaining of spermatozoa.|
|Mutations Made By|| |
David Garbers, U.T. Southwestern Medical Center
When maintained as a live colony, heterozygotes may be bred. Homozygous males are subfertile.
When using the 129S-Atp8b3tm1Gar/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007659 in your Materials and Methods section.
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