STOCK Tg(Col2a1-Npr2*B2)25-4Gar/J

Stock number: 007657
Research areas: Developmental Biology Research

Description

Mice that carry this transgene are significantly smaller than wildtype mice. At 10 days of age, expression of the transgenic “GC-B2” isoform is found in growth plate cartilage and cGMP levels of tail bones are decreased by 25%. These mice may be used in studies of endochondral ossification and bone development.

Detailed description

Mice that carry this transgene are viable, fertile and significantly smaller than wildtype mice. At 10 days of age, expression of the transgenic “GC-B2” isoform is found in growth plate cartilage and cGMP levels of tail bones are decreased by 25%. Skeletal growth, as measured by naso-anal length, is slightly reduced as compared with wildtype mice. These mice may be used in studies of endochondral ossification and bone development.

Development

A transgenic B geo expression construct incorporating the “GC-B2” isoform of the mouse gene (expressed in growth-plate chondrocytes) driven by the mouse Col2a1 (procollagen, type II, alpha 1) promoter/enhancer was introduced to B6SJLF1 embryos. Records for this strain are not well defined; it is believed to be on a mixed background composed of C57BL/6, 129 and SJL. This is line 25-4.

Allele

Symbol: Tg(Col2a1-Npr2*B2)25-4Gar
Name: transgene insertion 25-4, David L Garbers
MGI accession ID: MGI:3722765
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(Col2a1-Npr2*B2)25-4Gar
Marker name: transgene insertion 25-4, David L Garbers
Chromosome: UN
Strain of origin: C57BL/6 and SJL
Expressed genes: Npr2,natriuretic peptide receptor 2,mouse, laboratory
Molecular note: A transgenic B-geo expression construct incorporating the GC-B2 isoform of the mouse gene (expressed in growth-plate chondrocytes) driven by the mouse Col2a1 (procollagen, type II, alpha 1) promoter/enhancer was generated for microinjection. Lin 25-4 was developed.