Mice that carry this transgene are significantly smaller than wildtype mice. At 10 days of age, expression of the transgenic “GC-B2” isoform is found in growth plate cartilage and cGMP levels of tail bones are decreased by 25%. These mice may be used in studies of endochondral ossification and bone development.
David L. Garbers, U.T. Southwestern Medical Center
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Mice that carry this transgene are viable, fertile and significantly smaller than wildtype mice. At 10 days of age, expression of the transgenic “GC-B2” isoform is found in growth plate cartilage and cGMP levels of tail bones are decreased by 25%. Skeletal growth, as measured by naso-anal length, is slightly reduced as compared with wildtype mice. These mice may be used in studies of endochondral ossification and bone development.
A transgenic B geo expression construct incorporating the “GC-B2” isoform of the mouse gene (expressed in growth-plate chondrocytes) driven by the mouse Col2a1 (procollagen, type II, alpha 1) promoter/enhancer was introduced to B6SJLF1 embryos. Records for this strain are not well defined; it is believed to be on a mixed background composed of C57BL/6, 129 and SJL. This is line 25-4.
Expressed Gene | Npr2, natriuretic peptide receptor 2, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 25-4, David L Garbers |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | GCB2-Tgm; Tg(Col2a1-Npr2)254Gar; Tg(Col2a1-Npr2)25-4Gar |
Gene Symbol and Name | Tg(Col2a1-Npr2*B2)25-4Gar, transgene insertion 25-4, David L Garbers |
Gene Synonym(s) | |
Promoter | Col2a1, collagen, type II, alpha 1, mouse, laboratory |
Expressed Gene | Npr2, natriuretic peptide receptor 2, mouse, laboratory |
Strain of Origin | C57BL/6 and SJL |
Chromosome | UN |
Molecular Note | A transgenic B-geo expression construct incorporating the GC-B2 isoform of the mouse gene (expressed in growth-plate chondrocytes) driven by the mouse Col2a1 (procollagen, type II, alpha 1) promoter/enhancer was generated for microinjection. Lin 25-4 was developed. |
Mutations Made By | David Garbers, U.T. Southwestern Medical Center |
When maintained as a live colony, hemizygotes may be bred.
When using the STOCK Tg(Col2a1-Npr2*B2)25-4Gar/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007657 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Col2a1-Npr2)254Gar |
Frozen Mouse Embryo | STOCK Tg(Col2a1-Npr2*B2)25-4Gar/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Col2a1-Npr2*B2)25-4Gar/J | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Col2a1-Npr2*B2)25-4Gar/J | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Col2a1-Npr2*B2)25-4Gar/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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