B6;SJL-Tg(KRT14-Hr)551Cct/J

Stock number: 007622
Research areas: Dermatology Research

Description

-When young, these mice look similar to wildtype mice, but as they age, their coats become shorter and more sparse. Molecular analysis shows delayed development of sebaceous glands, accelerated hair cycle and less-differentiated epidermis. This strain may be useful as a model for skin and hair development studies.

Detailed description

Hemizygous mice are viable and fertile. When young, these mice look similar to wildtype mice, but as they age, their coats become shorter and more sparse. Molecular analysis shows delayed development of sebaceous glands, accelerated hair cycle and less-differentiated epidermis. RNA and protein expressed from the transgene are detected in skin as determined by Western and Northern analysis. This strain may be useful as a model for skin and hair development studies.

Development

A 3.5 kb fragment containing the coding sequence of the rat Hr cDNA was inserted between the human keratin 14 (KRT14) promoter/rabbit beta-globin intron and the KRT14 5' UTR/polyadenylation signal. The linearized transgene was microinjected into B6SJLF2 embryos. Transgenic mice were crossed with C57BL/6 one time by the donating laboratory.

Allele

Symbol: Tg(KRT14-Hr)551Cct
Name: transgene insertion 551, Catherine C Thompson
MGI accession ID: MGI:3715279
Generation method: Transgenic
Attributes: Inserted expressed sequence
Marker symbol: Tg(KRT14-Hr)551Cct
Marker name: transgene insertion 551, Catherine C Thompson
Chromosome: UN
Strain of origin: (C57BL/6 x SJL)F2
Expressed genes: Hr,HR, lysine demethylase and nuclear receptor corepressor,rat
Molecular note: A 3.5 kb fragment containing the coding sequence of the rat Hr cDNA was inserted between the human keratin 14 (KRT14) promoter/rabbit beta-globin intron and the KRT14 5' UTR/polyadenylation signal. Hr protein expression is high in the skin in early postnatal development, and remains above wild-type levels as development proceeds.