-The mice appear normal at birth and for about two weeks postnatally. Once the animals begin to shed, the hair is lost and does not grow back. They remain bald and over time, the skin becomes thicker and progressively more wrinkled. The degree of wrinkling is variable but can be severe; frequently the skin protrudes over the eyes and off the sides of the animals. Wrinkling is more severe than in the hairless rhino allele. This strain maybe be useful in studies of skin and hair.
Catherine Thompson, Wyeth Research
Homozygotes are viable and fertile, but optimal breeding is done with heterozygotes. The mice appear normal at birth and for about two weeks postnatally. Once the animals begin to shed, the hair is lost and does not grow back. They remain bald and over time, the skin becomes thicker and progressively more wrinkled. The degree of wrinkling is variable but can be severe; frequently the skin protrudes over the eyes and off the sides of the animals. Wrinkling is more severe than in the hairless rhino allele. Northern analysis of mRNA from back skin confirmed the absence of expression. This strain maybe be useful in studies of skin and hair.
A targeting vector was designed to replace exons 8-10 and a portion of exon 7 with a loxP-flanked PGKneo neomycin resistance cassette. The targeting vector was introduced to a 129S6/SvEvTac-derived embryonic stem (ES) cell line. Germline chimeras were crossed to C57BL/6 for seven generations by the donating laboratory.
|Allele Name||targeted mutation 1, Catherine C Thompson|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Hr, lysine demethylase and nuclear receptor corepressor|
|Strain of Origin||129/Sv|
|Molecular Note||Exons 8-10 were replaced with a floxed PGK-neo cassette. Southern blot confirmed recombination in mutant mice. Northern blot of mutant skin indicated lack of full-length mRNA and Western blot showed a lack of full-length protein in the skin of mutants.|
|Mutations Made By|| |
Catherine Thompson, Wyeth Research
When maintained as a live colony, heterozygous females may be bred with homozygous males. Of note, the previously reported information on these mice indicated that heterozygotes are more reliable breeders than homozygotes.
When using the B6.129S6-Hrtm1Cct/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007621 in your Materials and Methods section.
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