These PS1-floxed mice may be useful in generating conditional knockouts of Presenilin 1 for studying Alzheimer’s Disease.
Fred Van Leuven, Experimental Genetics Group - KULeuven
These mice possess loxP sites on either side of exon 7 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these "floxed" mice are bred to mice that express Cre recombinase, resulting offspring can have one of three resulting genotypes (only exon 7 deleted, only the neo selection cassette deleted, or both exon 7 and the neo selection cassette deleted) in the cre-expressing tissue(s). These PS1-floxed mice may be useful in generating conditional knockouts of Presenilin 1 for studying Alzheimer's Disease.
For example, when crossed to a strain expressing Cre recombinase in postnatal neurons (see Stock No. 006143), this mutant mouse strain may be useful in studies of amyloid plaque formation.
A loxP site flanked targeting vector containing a neomycin resistance gene was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 7 of the targeted gene, and another loxP site was inserted upstream of exon 7. This construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES). Correctly targeted ES cells were injected into recipient blastocysts. The donating investigator reports that these mice were then backcrossed to C57BL/6 for 7 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6J for one generation to establish the colony (see note below).
NOTE: A 27 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed a single loci that typed as homozygous for 129/P strain type (chromosome 12 approximately 9.8 cM) and three additional loci that were segregating for either C57BL/6J or 129/P strain type (chromosome 1 approximately 52.0 cM, chromosome 4 approximately 10.9 cM, and chromosome 8 approximately 4.4 cM). Therefore, these mice are determined to be on a mixed B6;129P genetic background (August 2008).
|Allele Name||targeted mutation 1, Fred Van Leuven|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Psen1tm1Vln; targeted mutation 1, Fred Van Leuven|
|Gene Symbol and Name||Psen1, presenilin 1|
|Gene Synonym(s)||alzheimer disease 3 homolog; S182; Ad3h; AD3; Ad3h; presenilin-1; PS-1; PS1; FAD; ACNINV3|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||Exon 7 was flanked by a single loxP site in intron 6 and floxed neo cassette in intron 7.|
|Mutations Made By|| |
Fred Van Leuven, Experimental Genetics Group - KULeuven
When maintaining a live colony, homozygous mice may be bred together.
When using the B6;129P-Psen1tm1Vln/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007605 in your Materials and Methods section.
|Heterozygous for Psen1<tm1Vln>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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