In these mutants the mouse immunoglobulin kappa chain, constant region was replaced with the human IGKC coding sequence.
Michel C Nussenzweig, The Rockefeller University
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Inserted expressed sequence, Humanized sequence) | Igkc | immunoglobulin kappa constant |
Mice homozygous for this targeted replacement of the mouse gene with the human homolog are viable and fertile and do not display any gross physical or behavioral abnormalities. Close to 50% of all B cells in heterozygous mice express human immunoglobulin kappa light chain constant region (Ck), detectable by immunostaining. This strain has been used to determine the extent of light chain receptor editing in the mouse by monitoring the replacement of kappa light chain that carries human constant region.
A targeting vector containing the human immunoglobulin kappa chain constant region (hCk, IGCK), a floxed neomycin cassette (5'), and a polymerase II promoter driven diptheria toxin gene (DTA)(3') was used to replace the homologous mouse locus. The vector was introduced to a 129Sv embryonic stem (ES) cell line. Mice carrying the mutation were crossed to a C57BL/6 background EIIA-Cre strain to remove the floxed neomycin cassette. This strain has was maintained on a mixed 129 and C57BL/6 background by the donating laboratory.
Expressed Gene | IGKC, immunoglobulin kappa constant, human |
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Site of Expression |
Allele Name | targeted mutation 1, Michel C Nussenzweig |
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Allele Type | Targeted (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | hCkappa; Igkappah |
Gene Symbol and Name | Igkc, immunoglobulin kappa constant |
Gene Synonym(s) | |
Expressed Gene | IGKC, immunoglobulin kappa constant, human |
Strain of Origin | Not Specified |
Chromosome | 6 |
Molecular Note | The mouse immunoglobulin kappa chain, constant region was replaced with the human IGKC coding sequence. A floxed neo selection cassette was subsequently removed from properly targeted mice by cre excision. |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the hCK mouse strain in a publication, please cite the originating article(s) and include JAX stock #007595 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Igk-C<tm1(IGKC)Mnz> |
Frozen Mouse Embryo | B6;129-Igkc<tm1(IGKC)Mnz>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Igkc<tm1(IGKC)Mnz>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Igkc<tm1(IGKC)Mnz>/J | $3373.50 |
Frozen Mouse Embryo | B6;129-Igkc<tm1(IGKC)Mnz>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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