B6;129-Igkc^tm1(IGKC)Mnz/J

Stock number: 007595
Product name: hCK
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

In these mutants the mouse immunoglobulin kappa chain, constant region was replaced with the human IGKC coding sequence.

Detailed description

Mice homozygous for this targeted replacement of the mouse gene with the human homolog are viable and fertile and do not display any gross physical or behavioral abnormalities. Close to 50% of all B cells in heterozygous mice express human immunoglobulin kappa light chain constant region (Ck), detectable by immunostaining. This strain has been used to determine the extent of light chain receptor editing in the mouse by monitoring the replacement of kappa light chain that carries human constant region.

Development

A targeting vector containing the human immunoglobulin kappa chain constant region (hCk, IGCK), a floxed neomycin cassette (5'), and a polymerase II promoter driven diptheria toxin gene (DTA)(3') was used to replace the homologous mouse locus. The vector was introduced to a 129Sv embryonic stem (ES) cell line. Mice carrying the mutation were crossed to a C57BL/6 background EIIA-Cre strain to remove the floxed neomycin cassette. This strain has was maintained on a mixed 129 and C57BL/6 background by the donating laboratory.

Allele

Symbol: Igkc^tm1(IGKC)Mnz
Name: targeted mutation 1, Michel C Nussenzweig
MGI accession ID: MGI:3690646
Generation method: Targeted
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Igkc
Marker name: immunoglobulin kappa constant
Chromosome: 6
Strain of origin: Not Specified
Expressed genes: IGKC,immunoglobulin kappa constant,human
Molecular note: The mouse immunoglobulin kappa chain, constant region was replaced with the human IGKC coding sequence. A floxed neo selection cassette was subsequently removed from properly targeted mice by cre excision.