129S4.129S7-Npy5r^tm1Rpa/J

Stock number: 007584
Product name: Y5R
Research areas: Diabetes and Obesity Research, Neurobiology Research

Description

Homozygous mutants develop mild late-onset obesity and show enhanced sensitivity to kainate-induced seizures.

Detailed description

Mice homozygous for this targeted mutation are viable and fertile. Young mice feed and grow normally, but develop mild late-onset obesity characterized by increased body weight, food intake and adiposity. Obesity is more notable in males than in females. Their response to intracerebroventricular administration of neuropeptide Y (NPY) and related peptides is either reduced or absent. The mice also show enhanced sensitivity to kainate-induced seizures. Transcript is detected in whole brain of null mice at levels less than 10% that of wildtype mice as determined by RT-PCR. Although lacZ is incorporated in the mutant locus, it is not expressed well (or at all).

Allele

Symbol: Npy5r^tm1Rpa
Name: targeted mutation 1, Richard D Palmiter
MGI accession ID: MGI:3028279
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Marker symbol: Npy5r
Marker name: neuropeptide Y receptor Y5
Chromosome: 8
Strain of origin: 129S7/SvEvBrd-Hprt1⁺
Expressed genes: lacZ,beta-galactosidase,E. coli
Molecular note: 760bp from the coding region were replaced with lacZ and a neomycin resistance gene. The replaced sequence included the translation initiation codon and sequence coding for the first 5 transmembrane spanning domains. RT-PCR indicated that less than 10% of normal transcript levels were present and this was most likely the consequence of read-through from the neomycin resistance gene rather than wild-type transcript.