These mice harbor the reporter RorcγtGFP mutant and may be useful in studying immune system homeostasis, T cell repertoire selection, CD4/CD8 double positive thymocyte survival, lymphoid organogenesis, proinflammatory T-helper cell development, mucosal immunology, and the role of inflammatory disease in autoimmunity and cancer progression.
Dr. Dan R. Littman, New York University Medical Center
Genetic Background | Generation |
---|---|
N6+N3F9
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter, Null/Knockout) | Rorc | RAR-related orphan receptor gamma |
Starting at:
$255.00 Domestic price for female 4-week |
510.00 Domestic price for breeder pair |
Mice homozygous for this Rorc(γtGFP (or RORγt)GFP) mutant allele are viable and fertile. While Rorcγ mRNA is detected in liver in Rorc(γ)tGFP homozygotes, mRNA and protein for the thymus-specific isoform (Rorcγt) encoded by the targeted allele are not detected in the thymus. EGFP expression reports Rorc(γt) transcription in the thymi of adult Rorc(γt)GFP mice. Homozygous mice exhibit abnormal lymph node, Peyer's patch, and lymphoid tissue inducer (LTi) cell development. Mice with Rorcγt-deficient T cells lack tissue-infiltrating proinflammatory T-helper cells (Th17 cells), and are protected from induced autoimmune disease (EAE) on this genetic background.
The donating investigator also reports increased thymoma incidence in homozygotes, with 80-90% of homozygotes succumbing to thymomas by 12-14 weeks of age. It should be noted that the time to onset may vary due to vivarium-specific environmental factors. In the JAX Repository colony, we have observed that female homozygotes live to 9-11 weeks of age and most male homozygotes die before 8 months of age (October 2015). Some researchers observe a much earlier onset. These RorcγtGFP mutant mice may be useful in applications related to immune homeostasis, T cell repertoire selection, CD4/CD8 double positive (CD4+/CD8+) thymocyte survival, and the role of inflammatory disease in autoimmunity and cancer progression.
A targeting vector was designed to insert the coding sequence of Enhanced Green Fluorescent Protein (EGFP), followed by a loxP-flanked neomycin resistance cassette, into "exon 1γt" directly downstream of the ATG translational start codon of the targeted gene. The construct was electroporated into the 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts, and chimeras were bred to unspecified mice to generate Rorc(γt)GFPneo heterozygotes. These mice were then crossed with a Cre-deleter strain (unspecified genetic background) to excise the neo cassette. The donating investigator reported that the resulting Rorc(γt)GFP pups were then backcrossed to C57BL/6 (see SNP note below) for at least five generations prior to arrival at The Jackson Laboratory.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, at least 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Expressed Gene | GFP, Green Fluorescent Protein, |
---|---|
Site of Expression | EGFP expression is reported in the thymus (cortex) of adult mice. EGFP is expressed in double-positive thymocytes, with small amounts of EGFP in CD4+ and CD8+ single-positive thymocytes. |
Allele Name | targeted mutation 2, Dan R Littman |
---|---|
Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Roc(gt)gfp; Rorc(gammat)-; Rorc(gammat)-EGFP; RorcGFP; RORgammat; RORgammat-EGFP |
Gene Symbol and Name | Rorc, RAR-related orphan receptor gamma |
Gene Synonym(s) | |
Expressed Gene | GFP, Green Fluorescent Protein, |
Site of Expression | EGFP expression is reported in the thymus (cortex) of adult mice. EGFP is expressed in double-positive thymocytes, with small amounts of EGFP in CD4+ and CD8+ single-positive thymocytes. |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 3 |
Molecular Note | An isoform-specific disruption was generated by inserting a cassette containing eGFP downstream of the start codon of exon 1gammat. The targeted exon is the third exon of the gene, serves as an alternative first exon for the gammat transcript, and is not included in the gamma transcript. RT-PCR analysis of hepatic RNA obtained from homozygous mutant mice identified gamma transcript but not the targted gammat transcript. The eGFP sequence included a stop codon but no splice sites or polyadenylation signals. A floxed neo cassette included in the targeting vector was excised from the germline via cre-mediated recombination. |
Mutations Made By | Dr. Dan Littman, New York University Medical Center |
Although homozygous mice are fertile, they have increased thymoma incidence.
The donating investigator reports 80-90% of homozygotes succumb to thymomas by 12-14 weeks of age. It should be noted that the time to onset may vary due to vivarium-specific environmental factors. In the JAX Repository colony, we have observed that female homozygotes live to 9-11 weeks of age and most male homozygotes die before 8 months of age (October 2015). Some researchers observe a much earlier onset.
The Jackson Laboratory Repository maintains its live colony by breeding heterozygous mice with homozygous mice.
When using the Rorc(γt)-EGFP mouse strain in a publication, please cite the originating article(s) and include JAX stock #007572 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-Type for Rorc<tm2Litt> |
Frozen Mouse Embryo | B6.129P2(Cg)-Rorc<tm2Litt>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2(Cg)-Rorc<tm2Litt>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129P2(Cg)-Rorc<tm2Litt>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129P2(Cg)-Rorc<tm2Litt>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.