B6SJL-Bmper^tm1Emdr/J

Stock number: 007554
Product name: CV2 KO
Research areas: Developmental Biology Research

Description

Mice homozygous for this knockout exhibit abnormal lung and skeleton development and die at birth.

Detailed description

Homozygous mutant mice die at birth due to respiratory failure. They display extensive skeletal abnormalities. At a lower penetrance, mutants show microphthalmia and exencephaly. RT-PCR was used to confirm expression in mouse embyronic fibroblasts.

Development

A targeting vector was used to place an in-frame lacZ reporter gene in the first exon of the gene at the initiator methionine and insert a neomycin resistance cassette downstream of lacZ. The targeting vector was introduced to LW1 129S4/SvJae-derived embryonic stem (ES) cells. This line has been backcrossed to B6SJLF1/J for 17 generations by the donating laboratory.

Allele

Symbol: Bmper^tm1Emdr
Name: targeted mutation 1, E M de Robertis
MGI accession ID: MGI:3711028
Generation method: Targeted
Attributes: Reporter; Null/Knockout
Synonyms: CV2; CV-2
Marker symbol: Bmper
Marker name: BMP-binding endothelial regulator
Marker synonyms: 3110056H04Rik; Crim3; crossveinless-2; Cv2; CV-2; RGD1563373
Chromosome: 9
Strain of origin: 129S4/SvJae
Expressed genes: lacZ,beta-galactosidase,E. coli
Molecular note: A targeting vector was used to place an in-frame LacZ reporter gene in the first exon of the gene at the initiator methionine deleting exon 2 and insert a neomycin resistance cassette downstream of LacZ.