Mice homozygous for this knockout exhibit abnormal lung and skeleton development and die at birth.
Edward De Robertis, University of California, Los Angeles
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Null/Knockout) | Bmper | BMP-binding endothelial regulator |
Homozygous mutant mice die at birth due to respiratory failure. They display extensive skeletal abnormalities. At a lower penetrance, mutants show microphthalmia and exencephaly. RT-PCR was used to confirm expression in mouse embyronic fibroblasts.
A targeting vector was used to place an in-frame lacZ reporter gene in the first exon of the gene at the initiator methionine and insert a neomycin resistance cassette downstream of lacZ. The targeting vector was introduced to LW1 129S4/SvJae-derived embryonic stem (ES) cells. This line has been backcrossed to B6SJLF1/J for 17 generations by the donating laboratory.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression |
Allele Name | targeted mutation 1, E M de Robertis |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | CV2; CV-2 |
Gene Symbol and Name | Bmper, BMP-binding endothelial regulator |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Strain of Origin | 129S4/SvJae |
Chromosome | 9 |
Molecular Note | A targeting vector was used to place an in-frame LacZ reporter gene in the first exon of the gene at the initiator methionine deleting exon 2 and insert a neomycin resistance cassette downstream of LacZ. |
Mutations Made By | Edward De Robertis, University of California, Los Angeles |
When maintaining a live colony, these mice are bred as heterozygotes. Homozygotes have a perinatal lethal phenotype.
When using the CV2 KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #007554 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Bmper<tm1Emdr> |
Frozen Mouse Embryo | B6SJL-Bmper<tm1Emdr>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6SJL-Bmper<tm1Emdr>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6SJL-Bmper<tm1Emdr>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6SJL-Bmper<tm1Emdr>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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