C57BL/6-Tg(Sry-EGFP)92Ei Chr Y^AKR/J/EiJ

Stock number: 007265
Product name: Sry-EGFP
Research areas: Developmental Biology Research, Neurobiology Research, Research Tools

Description

These transgenic mice express EGFP under the control of the Sry promoter in fetal gonads similarly to endogenous Sry.

Detailed description

EGFP under the control of the Sry promoter is expressed in fetal gonads similarly to endogenous Sry, first in cells located centrally in the genital ridge and then later in cells located at the cranial and caudal poles. EGFP is expressed exclusively in pre-Sertoli cells in the urogenital ridge.

Development

Tg(Sry-EGFP)92Ei was generated via random insertion after micro-injection into the pronucleus of C57BL/6J fertilized eggs. The resulting transgenic strain, C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ (Stock No. 003927), was bred to homozygosity and a female was bred to a male C57BL/6J-Chr Y^AKR/J/EiJ (Stock No. 007250) when that consomic was at generation N56. The progeny were backcrossed to homozygous C57BL/6J-Tg(Sry-EGFP)92Ei females until generation N15. In 2007 sperm were cryopreserved from a homozygous male at generation N15F2.

Allele

Symbol: Tg(Sry-EGFP)92Ei
Name: transgene insertion 92, Eva M Eicher
MGI accession ID: MGI:3703730
Generation method: Transgenic
Attributes: Reporter
Marker symbol: Tg(Sry-EGFP)92Ei
Marker name: transgene insertion 92, Eva M Eicher
Chromosome: UN
Strain of origin: C57BL/6J
Expressed genes: GFP,Green Fluorescent Protein,
Site of expression: Fluorescence is detected in pre-sertoli and pre-granulosa cells of the genital ridge, expression is stronger in homozygotes. Expression is also detected in tyrosine hydroxylase-expressing neurons of the substantia nigra of the midbrain and the medial mammillary bodies of the hypothalamus in males but not females.
Molecular note: The transgenic vector was composed of the Sry promoter region from bp 542-8304 inserted upstream of the EGFP-1 coding region. The construct contains two SV40 early mRNA polyadenylation signal in place of the endogenous Sry 3' UTR. Expression of EGFP indicates that the spatial expression of EGFP mRNA and protein is similar to endogenous Sry.