This strain expresses Cre-recombinase in the male germ line.
Read More +Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
Embryonic stem cells containing recombinase transgenes that are expressed in the male germ line, but not in other tissues or in the embryonic stem cells themselves, substantially simplify the production of subtle or conditional mutations in mice. This strain shows that transgenes comprised of the mouse protamine 1 promoter and the Cre recombinase coding sequence mediate the efficient recombination of a Cre target transgene in the male germ line, but not in other tissues. This system can be used for reducing the time, effort, and resources required to produce homologously recombined alleles in mice that have been secondarily rearranged by site-specific recombinase.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The PrmCre transgene was designed with the mouse protamine 1 promoter upstream of a modified Cre recombinase coding sequence (modified to contain a consensus translation start site, 11 codons for a human c-myc epitope, 7 codons for a minimal simian virus 40 nuclear localization signal, and a polyadenylation signal). This transgene was microinjected into pronuclei of fertilized 129/SvJae oocytes, and the resulting transgenic offspring were bred to 129/SvJae mice. Founder line 58 mice (with a single copy of the transgene) were backcrossed to 129Sv/Jae for many generations prior to arrival at The Jackson Laboratory (as Stock No. 003328. Upon arrival, some mice were backcrossed to C57BL/6EiJ (Stock No. 000924) to generate this congenic colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | male germ line |
Allele Name | transgene insertion 58, Stephen O'Gorman |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | Prm1cre; PrmCre; Prm-cre; Pro Cre; Tg(Prm-cre)580 g; TgNPro:Cre |
Gene Symbol and Name | Tg(Prm-cre)58Og, transgene insertion 58, Stephen O'Gorman |
Gene Synonym(s) | |
Promoter | Prm1, protamine 1, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | male germ line |
Strain of Origin | 129S4/SvJae |
Chromosome | UN |
Molecular Note | This transgene expresses Cre recombinase under the control of the mouse protamine (Prm1) promoter. The transgene directs expression of Cre recombinase in the male germ cells and not in other tissues. |
When maintaining a live colony, hemizygous mice may be bred to wildtype (non-carrier) mice or C57BL/6EiJ inbred mice (Stock No. 000924).
When using the B6Ei.129S4-Tg(Prm-cre)58Og/EiJ mouse strain in a publication, please cite the originating article(s) and include JAX stock #007252 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Prm-cre)58Og |
Frozen Mouse Embryo | B6Ei.129S4-Tg(Prm-cre)58Og/EiJ | $2595.00 |
Frozen Mouse Embryo | B6Ei.129S4-Tg(Prm-cre)58Og/EiJ | $2595.00 |
Frozen Mouse Embryo | B6Ei.129S4-Tg(Prm-cre)58Og/EiJ | $3373.50 |
Frozen Mouse Embryo | B6Ei.129S4-Tg(Prm-cre)58Og/EiJ | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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