Liver-specific expression of the transgene is exhibited in this strain of mice as determined by RT-PCR. When crossed with a F5 deficient mouse (Stock No. 004078), homozygous knockout/hemizygous transgenic mice have a plasma F5 level that is ~45% that of wildtype mice, no platelet F5, slightly prolonged bleeding times, and show transgene rescue of both mid-embryonic lethality and perinatal hemorrhage phenotypes associated with F5 knockouts.
David Ginsburg, University of Michigan
Genetic Background | Generation |
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Allele Type |
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Transgenic (Inserted expressed sequence) |
Liver-specific expression of the transgene is exhibited in this strain of mice as determined by RT-PCR. When crossed with a F5 targeted mouse (Stock No. 004078), homozygous knockout/hemizygous transgenic mice are grossly normal and show no evidence of spontaneous hemorrhage; compound mutants have a plasma F5 level that is ~45% that of wildtype mice, no platelet F5, slightly prolonged bleeding times, and show transgene rescue of both mid-embryonic lethality and perinatal hemorrhage phenotypes associated with F5 knockouts.
A mouse Alb (albumin) promoter drives expression of the mouse F5 (FV) transgene including the entire coding sequence. An internal ribosome entry site sequence (IRES) followed by an enhanced green fluorescent protein (EGFP) coding sequence and a bacterial kanamycin resistant gene (KAN) were also built into the cassette. A chimeric intron with optimized splice signals from the pCI expression vector was also introduced upstream of the F5 cDNA in the transgene cassette to enhance gene expression. No EGFP expression is observed. The vector was injected into fertilized oocytes created by mating B6SJLF1 or C57BL/6 female mice with B6SJLF1 male mice. This line has been backcrossed minimally (perhaps twice) to C57BL/6 by the donating laboratory.
Expressed Gene | F5, coagulation factor V, mouse, laboratory |
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Site of Expression |
Allele Name | transgene insertion 2, David Ginsburg |
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Allele Type | Transgenic (Inserted expressed sequence) |
Allele Synonym(s) | AlbfvC |
Gene Symbol and Name | Tg(Alb-F5)2Dgi, transgene insertion 2, David Ginsburg |
Gene Synonym(s) | |
Promoter | Alb, albumin, mouse, laboratory |
Expressed Gene | F5, coagulation factor V, mouse, laboratory |
Strain of Origin | (C57BL/6 x SJL)F1 or C57BL/6 x (C57BL/6 x SJL)F1 |
Chromosome | UN |
Molecular Note | The transgenic construct has the mouse albumin promoter driving expression of the murine F5 (FV) gene including the entire coding sequence. An IRES-EGFP cassette with a bacterial gene wasinserting after the F5 coding sequence. A chimeric intron with optimized splice signals was introduced upstream of the F5 cDNA enhance gene expression. Transgenic F5 expression is restricted to the liver. EGFP expression is not detected. |
Mutations Made By | David Ginsburg, University of Michigan |
When maintaining a live colony, hemizygous mice may be bred to wildtype siblings, or to B6SDJLF1/J mice (Stock No. 100012).
When using the B6;SJL-Tg(Alb-F5)2Dgi/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007244 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Alb-F5)2Dgi |
Frozen Mouse Embryo | B6;SJL-Tg(Alb-F5)2Dgi/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;SJL-Tg(Alb-F5)2Dgi/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;SJL-Tg(Alb-F5)2Dgi/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;SJL-Tg(Alb-F5)2Dgi/J Frozen Embryo | $3373.50 |
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