B6.Cg-Tg(Alb-PLG)1Dgi/J

Stock number: 007238
Research areas: Hematological Research

Description

The level of PLG in the plasma of these mice expressing the human plasminogen gene under the control of a mouse albumin promoter is approximately 16% of the level in control human plasma. Mice exhibit increased susceptibility to group A streptococci (GAS). Although enhanced green fluorescent protein (EGFP) is incorporated in the transgene, it is not expressed.

Detailed description

The level of PLG in the plasma of these mice expressing the human plasminogen gene under the control of a mouse albumin promoter is approximately 16% of the level in control human plasma. Mice exhibit increased susceptibility to group A streptococci (GAS). Although enhanced green fluorescent protein (EGFP) is incorporated in the transgene, it is not expressed.

Development

A transgenic BAC was designed to place human plasminogen (PLG) and green fluorescent protein (EGFP) under the control of a mouse albumin promoter (Alb). A chimeric intron with optimized splice signals from the pCI expression vector was placed upstream of the PLG cDNA. An FRT site and PGK-Tn5-Neo cassette were placed downstream of EGFP. The whole region, with the exception of Alb sequences, was flanked by loxP sites oriented in opposite directions. The PLG BAC DNA was isolated and microinjected into fertilized oocytes. Pronuclear fertilized eggs obtained by mating (C57BL6/J x SJL/J)F1 or C57BL/6 female mice with (C57BL6/J x SJL/J) F1 male mice were used for injection. This line has been backcrossed to C57BL/6 fifteen of more times by the donating laboratory. EGFP expression is not seen.

Allele

Symbol: Tg(Alb-PLG)1Dgi
Name: transgene insertion 1, David Ginsburg
MGI accession ID: MGI:3763545
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(Alb-PLG)1Dgi
Marker name: transgene insertion 1, David Ginsburg
Chromosome: UN
Strain of origin: (C57BL/6J x SJL/J)F1 x (C57BL/6J x SJL/J)F1 or (C57BL/6 x (C57BL/6J x SJL/J)F1)
Expressed genes: PLG,plasminogen,human
Molecular note: A cassette containing the human PLG cDNA from nucleotide -13 (A of ATG initiation codon as +1) to +2476 (including the entire coding sequence) was followed by the green fluorescent protein (EGFP) coding sequence, preceded by an internal ribosome entry site sequence (IRES) and a bacterial kanamycin-resistant gene (KAN). A chimeric intron with optimized splice signals from the pCI expression vector was placed upstream of the PLG cDNA. Homologous recombination between the transgene targeting construct and the murine Alb BAC was carried out in E. coli, replacing the murine Alb gene (from nucleotide -12 to exon 12) by the transgene cassette. EGFP expression is not detected.