These mice possess loxP sites on either side of exon 3 of the targeted gene. Mice that are homoozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele.
A targeting vector was designed to flank exon 3 with loxP sites, insert an additional loxP site 3' of the exon, and place a floxed neomycin cassette between exons 2 and 3. The targeting vector was introduced to a 129X1/Sv-derived embryonic stem (ES) cells. Targeted clones were injected into blastocysts and chimeric mice were used to generate heterozygous mice.
Heterozygotes were crossed to a FVB background strain of mice expressing the Cre recombinase gene under the control of the adenovirus EIIa promoter. Animals that retained the floxed exon 3 (and additional 3' loxP site), but lost the floxed neomycin cassette were selected for this line. This line was backcrossed to C57BL/6 at least six times by the donating laboratory.
|Allele Name||targeted mutation 2, David Ginsburg|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||Adamts13/delta Neo; Adamts13conditional|
|Gene Symbol and Name||Adamts13, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 13|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||Exon 3 of Adamts13 was flanked with LoxP sequences (plus an additonal LoxP located 511 bp 3'). In addition, a floxed neomycin cassette was inserted between exons 2 and 3.|
|Mutations Made By|| |
David Ginsburg, University of Michigan
When maintained as a live colony, homozygous animals may be bred.
When using the B6.129X1(FVB)-Adamts13tm2Dgi/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007236 in your Materials and Methods section.