STOCK Sebox^tm1Clc/J

Stock number: 007230

Description

These mice carry a targeted mutation of the Sebox (SEBOX homeobox) gene. No mutant phenotype has been observed in adult retinas examined using gene expression markers, or in electroretinograms under scotopic and photopic conditions. RNA in situ hybridization has been used to confirm the absence of gene expression.

Detailed description

No mutant phenotype has been observed in adult retinas examined using gene expression markers, or in electroretinograms under scotopic and photopic conditions. RNA in situ hybridization has been used to confirm the absence of gene expression.

Development

A targeting vector was used to flank the entire gene with loxP sites and insert a FRT-flanked PGK-neomycin resistance cassette in reverse orientation. The vector was injected into the 129S6/SvEvTac-derived TC1 embryonic stem (ES) cell line. Minimal crossing to C57BL/6 was carried out. The gene was removed by breeding mice to a an FVB background strain in which germline Cre expression was driven by a beta-actin (Actb) promoter. To remove the FRT-flanked neomycin cassette, the mice were mated to a 129S4 background mouse expressing FLP1 recombinase under the direction of a Gt(ROSA)26Sor promoter. One loxP and one FRT site remain in the knockout allele. Selective breeding was done to remove the FLP1 recombinase and the Pde6b^rd1 allele (found in FVB).

Allele

Symbol: Sebox^tm1Clc
Name: targeted mutation 1, Constance L Cepko
MGI accession ID: MGI:3708903
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Sebox
Marker name: SEBOX homeobox
Chromosome: 11
Strain of origin: 129S6/SvEvTac
Molecular note: A targeting vector was used to flank the entire gene with loxP sites and insert a Frt-flanked PGK-neomycin resistance cassette in reverse orientation. The gene was deleted by crossing these mice to a germline Cre strain, and subsequently mated to a line of germline Flp recombinase expressing mice, leaving a single loxP and an Frt site. No gene expression is detected by RNA-ISH.