These mice carry a targeted mutation of the Sebox (SEBOX homeobox) gene. No mutant phenotype has been observed in adult retinas examined using gene expression markers, or in electroretinograms under scotopic and photopic conditions. RNA in situ hybridization has been used to confirm the absence of gene expression.
Connie Cepko, Harvard Medical School, HHMI
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Sebox | SEBOX homeobox |
No mutant phenotype has been observed in adult retinas examined using gene expression markers, or in electroretinograms under scotopic and photopic conditions. RNA in situ hybridization has been used to confirm the absence of gene expression.
A targeting vector was used to flank the entire gene with loxP sites and insert a FRT-flanked PGK-neomycin resistance cassette in reverse orientation. The vector was injected into the 129S6/SvEvTac-derived TC1 embryonic stem (ES) cell line. Minimal crossing to C57BL/6 was carried out. The gene was removed by breeding mice to a an FVB background strain in which germline Cre expression was driven by a beta-actin (Actb) promoter. To remove the FRT-flanked neomycin cassette, the mice were mated to a 129S4 background mouse expressing FLP1 recombinase under the direction of a Gt(ROSA)26Sor promoter. One loxP and one FRT site remain in the knockout allele. Selective breeding was done to remove the FLP1 recombinase and the Pde6brd1 allele (found in FVB).
Allele Name | targeted mutation 1, Constance L Cepko |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Sebox, SEBOX homeobox |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 11 |
Molecular Note | A targeting vector was used to flank the entire gene with loxP sites and insert a Frt-flanked PGK-neomycin resistance cassette in reverse orientation. The gene was deleted by crossing these mice to a germline Cre strain, and subsequently mated to a line of germline Flp recombinase expressing mice, leaving a single loxP and an Frt site. No gene expression is detected by RNA-ISH. |
When maintaining a live colony, heterozygous mice may be bred together or to wildtype siblings.
When using the STOCK Seboxtm1Clc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007230 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-type for Sebox<tm1Clc> |
Frozen Mouse Embryo | STOCK Sebox<tm1Clc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Sebox<tm1Clc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Sebox<tm1Clc>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Sebox<tm1Clc>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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