Mice with this X-linked floxed mutation of the methyl CpG binding protein 2 gene may be useful in neurological and developmental studies of Rett syndrome.
Adrian Bird, University of Edinburgh
Genetic Background | Generation |
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N7F2
|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Mecp2 | methyl CpG binding protein 2 |
These mice possess two functional loxP sites flanking exons 3-4 of the targeted gene on the X chromosome (the donating investigator reports that the middle loxP site is non-functional). Homozygous females and hemizygous males are viable and fertile. Northern blot analysis showed the expected mature transcript from the Mecp2lox locus as well as a transcript in which the beta-globin intron was unspliced. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exons 3-4 deleted in the cre-expressing tissue(s). Mice with this X-linked floxed mutation may be useful in neurological and developmental studies of Rett syndrome.
For example, when crossed to a strain expressing Cre recombinase in nervous tissue (see Stock No. 003771), this mutant mouse strain develops a neurological phenotype that mimics Rett syndrome.
When crossed to a strain expressing Cre recombinase in GABAergic neurons (see Stock No. 017535), these mice exhibit behaviors common to those seen in Rett Syndrome and Autism Spectrum Disorders.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Allele Name | targeted mutation 1, Adrian Bird |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Mecp2lox |
Gene Symbol and Name | Mecp2, methyl CpG binding protein 2 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | X |
Molecular Note | Insertion of a neomycin resistance cassette into the gene introduced loxP sites that flank exons 3 and 4, and added an intron and polyadenylation signal from the human beta globin gene. From the mutated allele, Northern blot analysis detected the wild type mature transcript and also a transcript in which the beta globin intron was unspliced. |
Mutations Made By | Adrian Bird, University of Edinburgh |
Mutant mice were bred to C57BL/6J mice to generate this congenic strain. When maintaining the live congenic colony, females homozygous for this X-linked mutation can be bred with males hemizygous for this X-linked mutation.
When using the Mecp2lox mouse strain in a publication, please cite the originating article(s) and include JAX stock #007177 in your Materials and Methods section.
Service/Product | Description | Price |
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X linked - Heterozygous females and males wild-type for Mecp2<tm1Bird> |
Frozen Mouse Embryo | B6.129P2-Mecp2<tm1Bird>/J | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Mecp2<tm1Bird>/J | $2595.00 |
Frozen Mouse Embryo | B6.129P2-Mecp2<tm1Bird>/J | $3373.50 |
Frozen Mouse Embryo | B6.129P2-Mecp2<tm1Bird>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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