B6.129S4-Soat1^tm1Far/Pgn

Stock number: 007147
Product name: ACAT KO
Research areas: Cardiovascular Research, Internal/Organ Research, Metabolism Research

Description

Mice homozygous for the Soat1^tm1 targeted mutation in this strain display tissue specific decreases in cholesterol esterification and a 24% increase in HDL levels.

Detailed description

Phenotypically similar to B6.129S4-Soat1^tm1Far/J (Stock #003322), B6.129S4-Soat1^tm1Far/Pgn has a reduced congenic interval making the closely linked Apoa2 gene of C57BL/6 rather than 129S4 origin. The donating investigator indicates that HDL levels exhibit a 24% increase on high fat diet in this strain in contrast to the 73% increase observed in the original strain. Triglyceride levels are unchanged. This strain may be useful in atherosclerosis research.

Development

This strain is derived from B6.129S4-Soat1^tm1Far/J (003322) by further backcrossing to C57BL/6 reducing the congenic interval to a region defined by rs4222719 and D1Mit159. The reduced interval excludes the linked gene Apoa2 from the 129S4 derived region. Dr. Beverly Paigen performed the additional backcrosses at The Jackson Laboratory; the strain was cryopreserved in 2007.

Allele

Symbol: Soat1^tm1Far
Name: targeted mutation 1, Bob Farese
MGI accession ID: MGI:1857978
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Soat1
Marker name: sterol O-acyltransferase 1
Chromosome: 1
Strain of origin: 129S4/SvJae
Molecular note: Insertion of a neomycin cassette into a 5' early exon (amino acids 50-100). Reduced levels of a truncated mRNA transcript were detected by Northern blot on RNA from preputial glands. No protein was detected by immunoblot analysis in homogenates from preputial glands, ovaries and adrenals.