Mice homozygous for the Soat1tm1 targeted mutation in this strain display tissue specific decreases in cholesterol esterification and a 24% increase in HDL levels.
Read More +Genetic Background | Generation |
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000664 C57BL/6J |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Soat1 | sterol O-acyltransferase 1 |
Phenotypically similar to B6.129S4-Soat1tm1Far/J (Stock #003322), B6.129S4-Soat1tm1Far/Pgn has a reduced congenic interval making the closely linked Apoa2 gene of C57BL/6 rather than 129S4 origin. The donating investigator indicates that HDL levels exhibit a 24% increase on high fat diet in this strain in contrast to the 73% increase observed in the original strain. Triglyceride levels are unchanged. This strain may be useful in atherosclerosis research.
This strain is derived from B6.129S4-Soat1tm1Far/J (003322) by further backcrossing to C57BL/6 reducing the congenic interval to a region defined by rs4222719 and D1Mit159. The reduced interval excludes the linked gene Apoa2 from the 129S4 derived region. Dr. Beverly Paigen performed the additional backcrosses at The Jackson Laboratory; the strain was cryopreserved in 2007.
Allele Name | targeted mutation 1, Bob Farese |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Acact-; Acat-1- |
Gene Symbol and Name | Soat1, sterol O-acyltransferase 1 |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 1 |
Molecular Note | Insertion of a neomycin cassette into a 5' early exon (amino acids 50-100). Reduced levels of a truncated mRNA transcript were detected by Northern blot on RNA from preputial glands. No protein was detected by immunoblot analysis in homogenates from preputial glands, ovaries and adrenals. |
Mutations Made By | Robert Farese, Jr., Harvard University School of Public Health |
When using the ACAT KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #007147 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Soat1<tm1Far> |
Frozen Mouse Embryo | B6.129S4-Soat1<tm1Far>/Pgn | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Soat1<tm1Far>/Pgn | $2595.00 |
Frozen Mouse Embryo | B6.129S4-Soat1<tm1Far>/Pgn | $3373.50 |
Frozen Mouse Embryo | B6.129S4-Soat1<tm1Far>/Pgn | $3373.50 |
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