Phenotypically similar to B6.129S4-Soat1tm1Far/J (Stock #003322), B6.129S4-Soat1tm1Far/Pgn has a reduced congenic interval making the closely linked Apoa2 gene of C57BL/6 rather than 129S4 origin. The donating investigator indicates that HDL levels exhibit a 24% increase on high fat diet in this strain in contrast to the 73% increase observed in the original strain. Triglyceride levels are unchanged. This strain may be useful in atherosclerosis research.
This strain is derived from B6.129S4-Soat1tm1Far/J (003322) by further backcrossing to C57BL/6 reducing the congenic interval to a region defined by rs4222719 and D1Mit159. The reduced interval excludes the linked gene Apoa2 from the 129S4 derived region. Dr. Beverly Paigen performed the additional backcrosses at The Jackson Laboratory; the strain was cryopreserved in 2007.
|Allele Name||targeted mutation 1, Bob Farese|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Acact-; Acat-1-|
|Gene Symbol and Name||Soat1, sterol O-acyltransferase 1|
|Strain of Origin||129S4/SvJae|
|Molecular Note||Insertion of a neomycin cassette into a 5' early exon (amino acids 50-100). Reduced levels of a truncated mRNA transcript were detected by Northern blot on RNA from preputial glands. No protein was detected by immunoblot analysis in homogenates from preputial glands, ovaries and adrenals.|
|Mutations Made By|| |
Robert Farese, Jr., Harvard University School of Public Health