These interleukin 6 (IL6) knock-out mutant mice show defects in responses to various viruses and in inflammatory responses to tissue damage or infection.
IMR Colony, The Jackson Laboratory
Mice homozygous for the targeted mutation are viable and fertile. No gene product (mRNA) is detected by RT-PCR analysis of lipopolysaccharide (LPS) challenged macrophages. Bioassay and enzyme-linked immunosorbent assay (ELISA) analysis of serum from LPS-challenged homozygotes reveals no detectable protein activity. These interleukin 6 (IL6) mutant mice show defects in responses to various viruses and in inflammatory responses to tissue damage or infection.
Of note, IL6-mutant mice are available on different genetic backgrounds including mixed B6;129S2 (Stock No. 002254), C57BL/6J (Stock No. 002650), BALB/cByJ (Stock No. 007078), and NOD/ShiLtJ (Stock No. 029264).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The interleukin 6 targeted mutation was created by Manfred Kopf and Georges Kohler (Max Planck Institut Fur Immunbiologie, Freiburg Germany). A targeting vector was designed to place a neomycin resistance cassette into the first coding exon (exon 2) of the targeted gene. This construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Mutant mice on a C57BL/6 genetic background were sent to The Jackson Laboratory (as Stock No. 002650). Upon arrival, some mice were used for a speed congenic backcross to BALB/cByJ (Stock No. 001026) to generate this congenic strain (Stock No. 007078).
|Allele Name||targeted mutation 1, Manfred Kopf|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||IL-6 KO; IL-6-; Il6-; Il6tm1Koe; Il6tm1Kopf|
|Gene Symbol and Name||Il6, interleukin 6|
|Gene Synonym(s)||BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; ILg6; Ifnb2; Il-6; Il-6|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A neomycin selection cassette was inserted into exon 2. RT-PCR experiments on RNA derived from LPS-stimulated macrophages of homozygous mice demonstrated that no detectable transcript was produced from this allele. ELISA and bioassay experiments confirmed that no functional protein was made in homozygous mice.|
|Mutations Made By|| |
Dr. Manfred Kopf, ETH Zurich
Mutant mice were bred to BALB/cByJ inbred animals (using a speed congenic approach) to generate this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.
When using the IL-6<sup>-</sup> mouse strain in a publication, please cite the originating article(s) and include JAX stock #007078 in your Materials and Methods section.
|Heterozygous for Il6<tm1Kopf>|
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
order to provide the minimum number of animals, animals will ship within 25 weeks.
The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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