These interleukin 6 (IL6) knock-out mutant mice show defects in responses to various viruses and in inflammatory responses to tissue damage or infection.
IMR Colony, The Jackson Laboratory
Mice homozygous for the targeted mutation are viable and fertile. No gene product (mRNA) is detected by RT-PCR analysis of lipopolysaccharide (LPS) challenged macrophages. Bioassay and enzyme-linked immunosorbent assay (ELISA) analysis of serum from LPS-challenged homozygotes reveals no detectable protein activity. These interleukin 6 (IL6) mutant mice show defects in responses to various viruses and in inflammatory responses to tissue damage or infection.
Of note, IL6-mutant mice are available on different genetic backgrounds including mixed B6;129S2 (Stock No. 002254), C57BL/6J (Stock No. 002650), BALB/cByJ (Stock No. 007078), and NOD/ShiLtJ (Stock No. 029264).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The interleukin 6 targeted mutation was created by Manfred Kopf and Georges Kohler (Max Planck Institut Fur Immunbiologie, Freiburg Germany). A targeting vector was designed to place a neomycin resistance cassette into the first coding exon (exon 2) of the targeted gene. This construct was electroporated into 129S2/SvPas-derived D3 embryonic stem (ES) cells. Mutant mice on a C57BL/6 genetic background were sent to The Jackson Laboratory (as Stock No. 002650). Upon arrival, some mice were used for a speed congenic backcross to BALB/cByJ (Stock No. 001026) to generate this congenic strain (Stock No. 007078).
|Allele Name||targeted mutation 1, Manfred Kopf|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||IL-6-; Il6-; IL-6 KO; Il6tm1Koe; Il6tm1Kopf|
|Gene Symbol and Name||Il6, interleukin 6|
|Strain of Origin||129S2/SvPas|
|Molecular Note||A neomycin selection cassette was inserted into exon 2. RT-PCR experiments on RNA derived from LPS-stimulated macrophages of homozygous mice demonstrated that no detectable transcript was produced from this allele. ELISA and bioassay experiments confirmed that no functional protein was made in homozygous mice.|
|Mutations Made By|| |
Dr. Manfred Kopf, ETH Zurich
Mutant mice were bred to BALB/cByJ inbred animals (using a speed congenic approach) to generate this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.
When using the IL-6- mouse strain in a publication, please cite the originating article(s) and include JAX stock #007078 in your Materials and Methods section.