CByJ.129S7(B6)-Ifngr1^tm1Agt/J

Stock number: 007077
Product name: IFN-γR⁰
Research areas: Cancer Research, Immunology, Inflammation and Autoimmunity Research

Description

Mice homozygous for the Ifngr1^tm1 knock-out mutation have normal T cell responses but are defective in natural resistance, evidenced by an increased susceptibility to infection by Listeria monocytogenes and vaccinia virus. Mouse mutants involving this gene have been used in studies of Zika virus pathogenesis. Note: these mice carry the same allele as 'G129' mice, although on a congenic CByJ, not 129, genetic background.

Detailed description

Mice homozygous for the Ifngr1^tm1 targeted mutation are viable and fertile with no overt abnormalities. They have normal T cell responses but are defective in natural resistance, evidenced by an increased susceptibility to infection by Listeria monocytogenes and vaccinia virus.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A targeting vector containing a neo cassette was used to disrupt exon 5. The construct was electroporated into 129S7/SvEvBrd-Hprt1⁺ derived AB1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric male animals were crossed to 129SvEv female mice, and then backcrossed to C57BL/6J for 10 generations. The mice were then backcrossed to BALB/cByJ using a speed congenic protocol.

Allele

Symbol: Ifngr1^tm1Agt
Name: targeted mutation 1, Michel Aguet
MGI accession ID: MGI:1857286
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Ifngr1
Marker name: interferon gamma receptor 1
Chromosome: 10
Strain of origin: 129S7/SvEvBrd-Hprt1⁺
Molecular note: Insertion of a neomycin cassette into exon V, which encodes an extracellular membrane-proximal portion of the receptor.